pH-Sensitive Ratiometric Fluorescent Probe for Evaluation of Tumor Treatments

Materials

卷:12

Yingying Li¹  Zihua Wang¹  Junli Meng¹  Yi Hou¹  Peisen Zhang¹ 

[1] Key Laboratory of Colloid, Interface and Chemical Thermodynamics, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100190, China;

关键词: ratiometric fluorescence;    intracellular pH;    pharmacodynamic evaluation;   

DOI  :  10.3390/ma12101632

来源: DOAJ

【 摘 要 】

Determining therapeutic efficacy is critical for tumor precision theranostics. In order to monitor the efficacy of anti-cancer drugs (e.g., Paclitaxel), a pH-sensitive ratiometric fluorescent imaging probe was constructed. The pH-sensitive ratiometric fluorescent dye ANNA was covalently coupled to the N-terminal of the cell-penetrating TAT peptide through an amidation reaction (TAT-ANNA). The in vitro cellular experiments determined that the TAT-ANNA probe could penetrate the cell membrane and image the intracellular pH in real time. The in vivo experiments were then carried out, and the ratiometric pH response to the state of the tumor was recorded immediately after medication. The TAT-ANNA probe was successfully used to monitor the pharmacodynamics of anti-cancer drugs in vivo.

【 授权许可】

Unknown