Imaging of Fluoride Ion in Living Cells and Tissues with a Two-Photon Ratiometric Fluorescence Probe

Sensors

Xinyue Zhu¹  Jianxi Wang¹  Jianjian Zhang¹  Zhenjie Chen²  Haixia Zhang¹ 

[1] State Key Laboratory of Applied Organic Chemistry, College of Chemistry and Chemical Engineering, Lanzhou University, Lanzhou 730000, China; E-Mails:;Institute of Physiology, School of Basic Medical Sciences, Lanzhou University, Lanzhou 730000, China; E-Mails:

关键词: two-photon excited;    ratiometric fluorescence;    imaging;    fluoride;   

DOI  :  10.3390/s150101611

来源: mdpi

【 摘 要 】

A reaction-based two-photon (TP) ratiometric fluorescence probe Z2 has been developed and successfully applied to detect and image fluoride ion in living cells and tissues. The Z2 probe was designed designed to utilize an ICT mechanism between n-butylnaphthalimide as a fluorophore and tert-butyldiphenylsilane (TBDPS) as a response group. Upon addition of fluoride ion, the Si-O bond in the Z2 would be cleaved, and then a stronger electron-donating group was released. The fluorescent changes at 450 and 540 nm, respectively, made it possible to achieve ratiometric fluorescence detection. The results indicated that the Z2 could ratiometrically detect and image fluoride ion in living cells and tissues in a depth of 250 μm by two-photon microscopy (TPM).

【 授权许可】

CC BY   
© 2015 by the authors; licensee MDPI, Basel, Switzerland.