Elisa and HPLC analyses of deoxynivalenol in maize and wheat

Zbornik Matice Srpske za Prirodne Nauke

卷:2011

关键词: Deoxynivalenol;    ELISA;    HPLC;    maize;    wheat;   

DOI  :  10.2298/ZMSPN1120025M

来源: DOAJ

【 摘 要 】

Deoxynivalenol (DON) is a part of the family of mycotoxins calledtrichothecenes which are produced by a number of different Fusarium moldspecies. The presence of DON in 25 wheat and 25 maize samples was examined byEnzyme Linked Immunosorbent Assay (ELISA) and High Performance LiquidChromatography (HPLC) methods. The presence of DON was detected anddetermined in 5 (20%) maize and 6 (25%) wheat samples by both of the methods.Correlation between ELISA and HPLC results was established, with thecorrelation coefficients (r) of 0.9691 and 0.9735 for wheat and maizesamples, respectively. The results obtained by ELISA method weresignificantly higher than those obtained by HPLC method. This fact can beexplained by the presence of conjugated or masked mycotoxins in the samples,especially DON-3-glucoside (DON-3-Glc), which could not be determined by HPLCmethod due to the lack of external standards. Contrary to this, beinginsufficiently selective towards masked DON, ELISA method measures total DONcontent of a sample. According to the obtained results, ELISA can be used asa reliable screening method, but the confirmation of positive results must bedone by HPLC method.

【 授权许可】

Unknown