A protocol for metabolic characterization of human induced pluripotent stem cell-derived cardiomyocytes (iPS-CM)

MethodsX

卷:7

Yana Sandlers¹  Erica Fatica²  Rohan Shah²  Ryan Pearce²  Alisha House²  Jared Stergar² 

[1] Corresponding author.;

[2] Department of Chemistry, Cleveland State University, Cleveland, OH 44115, United States;

关键词: Induced pluripotent stem cell-derived cardiomyocytes;    Metabolic characterization;    GCMS;    LC–MS/MS;   

DOI  :  

来源: DOAJ

【 摘 要 】

Recent advances in human induced pluripotent stem cell-derived cardiomyocytes (iPSCM) field offer a novel platform for modeling cardiac metabolism, heart diseases drug candidates screening and cardiac toxicity assessments. These workflows require a fully functional characterization of iPSCMs. Here we report a step by step protocol for iPSCM metabolic characterization. The described assays cover analysis of small metabolites involved in a vital metabolic pathways.

【 授权许可】

Unknown