Macrophagic Inflammatory Response Next to Dental Implants with Different Macro- and Micro-Structure: An In Vitro Study

Applied Sciences

卷:11

Francesco Pera¹  Domenico Baldi²  Francesco Bagnasco²  Francesca Delucchi²  Maria Menini²  Paolo Pesce² 

[1] Department of Surgical Sciences, CIR-Dental School, University of Turin, 10100 Turin, Italy;

[2] Division of Prosthodontics and Implant Prosthodontics, Department of Surgical Sciences (DISC), University of Genova, 16100 Genova, Italy;

关键词: dental implants;    biomaterials;    implant surface;    endotoxins;    osteoimmunology;    inflammatory response;   

DOI  :  10.3390/app11125324

来源: DOAJ

【 摘 要 】

(1) Background: Intrinsic characteristics of the implant surface and the possible presence of endotoxins may affect the bone–implant interface and cause an inflammatory response. This study aims to evaluate the possible inflammatory response induced in vitro in macrophages in contact with five different commercially available dental implants. (2) Methods: one zirconia implant NobelPearl^® (Nobel Biocare) and four titanium implants, Syra^® (Sweden & Martina), Prama^® (Sweden & Martina), 3iT3^® (Biomet 3i) and Shard^® (Mech & Human), were evaluated. After 4 h of contact of murine macrophage cells J774a.1 with the implants, the total RNA was extracted, transcribed to cDNA and the gene expression of the macrophages was evaluated by quantitative PCR (qPCR) in relation to the following genes: GAPDH, YWHAZ, IL1β, IL6, TNFα, NOS2, MMP-9, MMP-8 and TIMP3. The results were statistically analyzed and compared with negative controls. (3) Results: No implant triggered a significant inflammatory response in macrophages, although 3iT3 exhibited a slight pro-inflammatory effect compared to other samples. (4) Conclusions: All the samples showed optimal outcomes without any inflammatory stimulus on the examined macrophagic cells.

【 授权许可】

Unknown