Gelatin/bioactive glass composite scaffold for promoting the migration and odontogenic differentiation of bone marrow mesenchymal stem cells

Polymer Testing

卷:93

Sainan Wang¹  Liju Xu²  Jilin Wu³  Yanmei Dong⁴  Guibin Huang⁵ 

[1] Beijing Key Laboratory of Digital Stomatology, Beijing, 100081, PR China;

[2] National Clinical Research Center for Oral Diseases &

[3] National Engineering Laboratory for Digital and Material Technology of Stomatology &

[4] Beijing National Laboratory for Molecular Sciences, CAS Research/Education Center for Excellence in Molecular Sciences, Institute of Chemistry, Chinese Academy of Sciences, Beijing, 100190, PR China;

[5] Department of Cariology and Endodontology, Peking University School and Hospital of Stomatology &

关键词: pH-neutral bioactive glass;    Composite scaffold;    Bone marrow mesenchymal stem cells;    Migration;    Odontogenic differentiation;    Pulp regeneration;   

DOI  :  

来源: DOAJ

【 摘 要 】

Recruiting periapical stem cells into the root canal and inducing their odontogenic differentiation are both vital for pulp regeneration. This study was aimed at developing a kind of bioactive composite scaffolds (G/PSC scaffolds) by combining the pH-neutral bioactive glass named PSC and gelatin. The incorporation of PSC endowed the scaffolds with bioactivity and enhanced its resistance to enzymatic hydrolysis in vitro while maintaining its high water absorption capacity. The G/PSC scaffold with a PSC content of 0.5 mg/mL in 3% gelatin solution showed the best overall performance, released large amounts of silicate ions and maintained a neutral pH after soaking in simulated body fluid (SBF). Human bone marrow mesenchymal stem cells (hBMMSCs) developed good cell morphology with affluent pseudopods on the G/PSC scaffold and infiltrated fairly deep into it. Additionally, hBMMSCs showed increased proliferation and mRNA expression levels of dentin sialophosphoprotein (DSPP), dentin matrix protein 1 (DMP-1), alkaline phosphatase (ALP), collagen type I (Col-I), osteocalcin (OCN), and runt-related transcription factor 2 (Runx2). The present results demonstrated that the G/PSC scaffold significantly promoted hBMMSC chemotaxis, proliferation and odontogenic differentiation. Therefore, the G/PSC scaffold is a promising material for pulp regeneration.

【 授权许可】

Unknown