IMMUNOHISTOCHEMICAL CHARACTERISATION OF LYMPHOID POPULATIONS IN RABBIT JEJUNAL PEYER’S PATCH

EUREKA: Life Sciences

Volodymyr Khomych¹  OlhaFedorenko¹ 

[1] National University of Life and Environmental Sciences of Ukraine;

关键词: rabbit;    jejunum;    lymphoid tissue;    peyer's patch;    cd3;    cd10;    cd20;    immunohistochemistry;   

DOI  :  10.21303/2504-5695.2020.001402

来源: DOAJ

【 摘 要 】

Rabbits are an important livestock animal species, which are used for their meat and fur. Nowadays they are also becoming more popular as pets. Furthermore, rabbits are commonly used in research, inter alia in immunological studies and for studying pathogenesis of human and animal diseases.The lymphoid tissue is abundant in the rabbit intestine and a lot of it is concentrated in Peyer's patches, the majority of which is located in the jejunum. Understanding of the rabbit Peyer's patches functions is essential for the prevention and treatment of their diseases. In order to enhance it, accurate knowledge of its lymphocyte population composition is needed. At present, the cellular composition of the rabbit gut-associated lymphoid tissue remains insufficiently studied. Therefore, the aim of our study was to establish the content and localization of lymphoid populations in the domestic rabbit jejunal Peyer’s patches. We performed immunohistochemical studies using monoclonal antibodies CD3, CD10 and CD20 (DAKO, Denmark) to identify the distribution of lymphoid populations in jejunal Peyer's patches of a mature domestic rabbit. The results, obtained in our study, complement the existing data and determine the main lymphoid populations in the rabbit jejunal Peyer's patches. The presented data are fundamental for further studies of the rabbit gut-associated lymphoid tissue.We found that lymphoid nodules of jejunal Peyer's patches contains a predominant CD20+ cell population (B cells). It also contains many CD10+ cells (precursors of T cells and B cells), which are mostly concentrated in the germinal centers of lymphoid nodules. In the diffuse form of the lymphoid tissue the vast majority of cells are CD3+ cells (T cells)

【 授权许可】

Unknown