Comparative evaluation of CC chemokine-induced migration of murine CD8α+ and CD8α− dendritic cells and their in vivo trafficking

Journal of Leukocyte Biology

Adrian E. Morelli⁴  Angus W. Thomson²  Bridget L. Colvin³  Audrey H. Lau³  Alison J. Logar¹ 

[1] Thomas E. Starzl Transplantation Institute and Departments of Thomas E. Starzl Transplantation Institute and Departments of Thomas E. Starzl Transplantation Institute and Departments of;Thomas E. Starzl Transplantation Institute and Departments of Surgery and Immunology, University of Pittsburgh Medical Center, Pennsylvania Thomas E. Starzl Transplantation Institute and Departments of Thomas E. Starzl Transplantation Institute and Departments of Surgery and Immunology, University of Pittsburgh Medical Center, Pennsylvania Surgery and Thomas E. Starzl Transplantation Institute and Departments of Surgery and Immunology, University of Pittsburgh Medical Center, Pennsylvania Immunology, University of Pittsburgh Medical Center, Pennsylvania Thomas E. Starzl Transplantation Institute and Departments of Surgery and Immunology, University of Pittsburgh Medical Center, Pennsylvania;Thomas E. Starzl Transplantation Institute and Departments of Immunology, University of Pittsburgh Medical Center, Pennsylvania Thomas E. Starzl Transplantation Institute and Departments of Thomas E. Starzl Transplantation Institute and Departments of Immunology, University of Pittsburgh Medical Center, Pennsylvania Immunology, University of Pittsburgh Medical Center, Pennsylvania Thomas E. Starzl Transplantation Institute and Departments of Immunology, University of Pittsburgh Medical Center, Pennsylvania;Thomas E. Starzl Transplantation Institute and Departments of Surgery and Thomas E. Starzl Transplantation Institute and Departments of Thomas E. Starzl Transplantation Institute and Departments of Surgery and Surgery and Thomas E. Starzl Transplantation Institute and Departments of Surgery and

关键词: leukocyte chemotaxis;    antigen-presenting cell;    lymphoid tissue;   

DOI  :  10.1189/jlb.1202613

学科分类：生理学

来源: Federation of American Societies for Experimental Biology

【 摘 要 】

Murine CD11c+CD8α− and CD11c+CD8α+ dendritic cells (DCs) differentially regulate T cell responses. Although specific chemokines that recruit immature (i) or mature (m) CD8α− DCs have been identified, little is known about the influence of chemokines on CD8α+ DCs. iDCs and mDCs isolated from spleens of fms-like tyrosine kinase 3 ligand-treated B10 mice were compared directly for migratory responses to a panel of CC chemokines or following local or systemic administration. In vitro assays were performed using Transwell® chambers. iDCs did not respond to any CC chemokines tested. Both subsets of mDCs migrated to CCL19 and CCL21, with consistently lower percentages of CD8α+ DCs migrating. Chemokine receptor mRNA and protein expression were analyzed, but no correlation between expression and function was demonstrated. In vivo trafficking of fluorochrome-labeled DCs (B10; H2b) was assessed by immunohistochemistry and by rare-event flow cytometric analysis of allogeneic recipient (BALB/c; H2d) draining lymph node (DLN) and spleen cells. Twenty-four hours after intravenous injection, chloromethylfluorescein diacetate-positive CD8α+ and CD8α− mDCs were detected by immunohistochemistry in spleens in similar numbers (that decreased over time). Following subcutaneous injection, both DC subsets were detected in DLN at 24 h, but only CD8α− DCs were evident by flow analysis at 48 h. Although CD8α+ DCs migrate from peripheral tissues to T cell areas of (allogeneic) secondary lymphoid organs, they appear to mobilize as mDCs and less efficiently than CD8α− mDCs.

【 授权许可】

Unknown