Metabolic fate and organ distribution of 13C-3′-sialyllactose and 13C-N-acetylneuraminic acid in wild-type mice – No evidence for direct incorporation into the brain

Journal of Functional Foods

卷:75

Silvia Rudloff¹  Christian Borsch²  Clemens Kunz³  Martina Reutzel³  Gunter Eckert³  Sabine Kuntz³  Sebastian P. Galuska³  Christina E. Galuska⁴ 

[1] Core Facility Metabolomics, Leibniz Institute for Farm Animal Biology (FBN), 18196 Dummerstorf, Germany;

[2] Department of Pediatrics, Justus-Liebig University Giessen, Feulgenstr.10-12, 35390 Giessen, Germany;

[3] Institute of Nutritional Sciences, Justus-Liebig University Giessen, 35390 Giessen, Germany;

[4] Institute of Reproductive Biology, Leibniz Institute for Farm Animal Biology (FBN), 18196 Dummerstorf, Germany;

关键词: 3′ sialyllactose;    N-acetylneuraminic acid;    Stable isotopes;    Metabolism;    Brain;    Wildtype mice;   

DOI  :  

来源: DOAJ

【 摘 要 】

Milk sialyllactose (SL) and sialic acids (SA) are considered to be crucial for brain composition and development. To investigate their metabolic fate, we administered 13C-labelled 3′SL (13C-3′SL) and 13C-N-acetylneuraminic acid (13C-Neu5Ac) to NMRI mice. From per oral and intravenous (i.v.) applications, an organ specific 13C-enrichment can be excluded. The 13C-enrichment after oral application (o.a.) was lowest in brain tissue and not detectable after i.v. in any organ. The presence of 13C-Neu5Ac in urine after the o.a. of both labelled components demonstrated that 13C-Neu5Ac was taken up by gut epithelial cells. Because plasma 13C-enrichment increased over time, when the oral 13C-bolus had reached the lower gastrointestinal tract, an involvement of intestinal epithelial cells and/or gut microbiota in the metabolism of 13C-3′SL and/or 13C-Neu5Ac could be assumed. Hence, SL or Neu5Ac might influence the gut brain axis by effects within the gastrointestinal tract rather than being directly incorporated into the brain.

【 授权许可】

Unknown