Genetic variability of elite barley genotypes for brazilian savanna irrigated systems based on RAPD markers

Bioscience Journal

卷:30

José Ricardo Peixoto¹  Flávio Capettini¹  Walter Quadros Ribeiro Júnior¹  Bernardo Coutinho de Almeida¹  Fábio Gelape Faleiro¹  Renato Fernando Amabile² 

[1] ;

[2] Embrapa Cerrados;

关键词: cevada;    diversidade genética;    melhoramento genético;    recursos genéticos;    RAPD;   

DOI  :  

来源: DOAJ

【 摘 要 】

The objective of this work was to characterize and quantify the genetic variability of 39 barley elite genotypes from a Brazilian working collection belonging to Embrapa, using RAPD (Random Amplified Polymorphic DNA) molecular markers. Genomic DNA samples were extracted from leaves of each genotype and 15 decamer primers were used to obtain RAPD molecular markers. Molecular markers were converted in a binary data matrix utilized to estimate genetic dissimilarities between genotypes and to realize grouping and dispersion graphic analysis. A total of 160 RAPD markers were obtained, making 10.7 markers medium per primer. From all the markers, 141 (88.12%) were polymorphic. Genetic dissimilarities varied from 0.049 to 0.337 among the genotypes. PFC 2004033 and Prestige cultivar showed biggest genetic dissimilarities to others genetic materials. Grouping and dispersion graphic analysis showed a clustering tendency between the Mexican and American genotypes. Another clustering tendency was also found concerning the six-rowed materials. Accessions developed and used in Brazil and also in Germany, UKand Australia have shown the greatest genetic dissimilarity among themselves, being considered promising options to increase the genetic base of breeding programs.

【 授权许可】

Unknown