Molecular characterization of papaya genotypes using AFLP markers

Revista Brasileira de Fruticultura

Eder Jorge de Oliveira¹  Jorge Luiz Loyola Dantas¹  Fabiana Moraes de Carvalho²  Aline dos Santos Silva³  Juliana Leles Costa³  Lucas Ferraz dos Santos³ 

[1]Embrapa Mandioca e Fruticultura

[2]Faculdade Maria Milza

[3]Universidade Federal do Recôncavo da Bahia

关键词: melhoramento;    recursos genéticos;    diversidade genética;    Carica papaya L;   

DOI  :  10.1590/S0100-29452011000300020

来源: DOAJ

【 摘 要 】

Due to the low genetic variability reported in the commercial plantations of papaya (Carica papaya L.), the objective of this study was analyze the genetic diversity of 32 genotypes including cultivars, landraces, inbred lines, and improved germplasm using the AFLP technique (Amplified Fragment Length Polymorphism). The genetic distance matrix was obtained using the Nei and Li genetic distance and clustering was performed using the unweighted pair-method with arithmetic mean (UPGMA). Using 11 combinations of EcoRI/MseI primers, 383 polymorphic bands were obtained. On average, 34.8 polymorphic bands were obtained per primer combination. Five clusters were formed. The traditional cultivar 'Sunrise' and the inbred line CMF-L30-08 were the closest genotypes, and the improved germplasm (CMF041) and landrace (CMF233) the most distant. The main papaya cultivars commercially grown in Brazil, as well as four inbred lines and three improved germplasm, were clustered together, however, were not grouped in the same branch. The genetic distance between the Sunrise and Golden cultivars was 0.329, and even arising from mutation and selection within the Sunrise variety, the Golden stores considerable genetic variability. Additional variability was observed in the inbred lines derived from papaya breeding program at Embrapa Cassava and Fruits.

【 授权许可】

Unknown