Replacing critical point drying with a low-cost chemical drying provides comparable surface image quality of glandular trichomes from leaves of Millingtonia hortensis L. f. in scanning electron micrograph

Applied Microscopy

卷:50

Olga Kostina¹  Lyudmila Muravnik¹  Raktim Bhattacharya²  Adinpunya Mitra²  Sulagna Saha² 

[1] Laboratory of Plant Anatomy and Morphology, Komarov Botanical Institute, Russian Academy of Sciences;

[2] Natural Product Biotechnology Group, Agricultural and Food Engineering Department, Indian Institute of Technology Kharagpur;

关键词: Scanning electron microscope;    Critical-point drying;    Chemical drying;    Hexamethyldisilazane;    Millingtonia hortensis;    Glandular trichomes;   

DOI  :  10.1186/s42649-020-00035-6

来源: DOAJ

【 摘 要 】

Abstract Sample preparation including dehydration and drying of samples is the most intricate part of scanning electron microscopy. Most current sample preparation protocols use critical-point drying with liquid carbon dioxide. Very few studies have reported samples that were dried using chemical reagents. In this study, we used hexamethyldisilazane, a chemical drying reagent, to prepare plant samples. As glandular trichomes are among the most fragile and sensitive surface structures found on plants, we used Millingtonia hortensis leaf samples as our study materials because they contain abundant glandular trichomes. The results obtained using this new method are identical to those produced via critical-point drying.

【 授权许可】

Unknown