Contraction-induced skeletal muscle FAT/CD36 trafficking and FA uptake is AMPK independent

Journal of Lipid Research

卷:52

J.B. Birk¹  A.J. Rose²  P. Schjerling²  P.H. Albers²  J. Jeppesen²  N. Dzamko³  G.R. Steinberg⁴  B. Kiens⁴ 

[1] Molecular Metabolic Control, German Cancer Research Center, Heidelberg, Germany;

[2] Copenhagen Muscle Research Center, Molecular Physiology Group, Section of Human Physiology, University of Copenhagen, Copenhagen, Denmark;

[3] Department of Exercise and Sport Science, and Institute of Sports Medicine, Bispebjerg Hospital, and Center for Healthy Aging, Faculty of Health Sciences, University of Copenhagen, Copenhagen, Denmark;

[4] St. Vincent's Institute of Medical Research and Department of Medicine, University of Melbourne, Melbourne, Australia;

关键词: fatty acid metabolism;    fatty acid translocase CD36;    trafficking;    AMP-dependent protein kinase;   

DOI  :  

来源: DOAJ

【 摘 要 】

The aim of this study was to investigate the molecular mechanisms regulating FA translocase CD36 (FAT/CD36) translocation and FA uptake in skeletal muscle during contractions. In one model, wild-type (WT) and AMP-dependent protein kinase kinase dead (AMPK KD) mice were exercised or extensor digitorum longus (EDL) and soleus (SOL) muscles were contracted, ex vivo. In separate studies, FAT/CD36 translocation and FA uptake in response to muscle contractions were investigated in the perfused rat hindlimb. Exercise induced a similar increase in skeletal muscle cell surface membrane FAT/CD36 content in WT (+34%) and AMPK KD (+37%) mice. In contrast, 5-aminoimidazole-4-carboxamide ribonucleoside only induced an increase in cell surface FAT/CD36 content in WT (+29%) mice. Furthermore, in the perfused rat hindlimb, muscle contraction induced a rapid (1 min, +15%) and sustained (10 min, +24%) FAT/CD36 relocation to cell surface membranes. The increase in cell surface FAT/CD36 protein content with muscle contractions was associated with increased FA uptake, both in EDL and SOL muscle from WT and AMPK KD mice and in the perfused rat hindlimb. This suggests that AMPK is not essential in regulation of FAT/CD36 translocation and FA uptake in skeletal muscle during contractions. However, AMPK could be important in regulation of FAT/CD36 distribution in other physiological situations.

【 授权许可】

Unknown