Hepatic amino acid‐dependent signaling is under the control of AMP‐dependent protein kinase

FEBS Letters

Dubbelhuis, Peter F¹  Meijer, Alfred J¹ 

[1] Department of Biochemistry, Academic Medical Center, University of Amsterdam, Meibergdreef 15, 1105 AZ Amsterdam, The Netherlands

关键词: Signal transduction;    AICAR;    AMP-dependent protein kinase;    Amino acid;    Mammalian target of rapamycin;    p70S6 kinase;    AICAR;    AICAriboside;    imidazole-4-carboxamide 1-β-D-ribofuranoside;    AMPK;    AMP-dependent protein kinase;    mTOR;    mammalian target of rapamycin;    PKB;    protein kinase B;   

DOI  :  10.1016/S0014-5793(02)02815-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

It has become increasingly clear in recent years that amino acids can stimulate a signal transduction pathway resulting in the phosphorylation of mammalian target of rapamycin downstream targets. We have now found that amino acid-dependent phosphorylation of p70S6 kinase and of S6 in hepatocytes is prevented when AMP-dependent protein kinase (AMPK) is activated by either the purine ribonucleoside analogue AICAriboside, fructose or glycerol. Insulin-dependent phosphorylation of protein kinase B is not affected by AMPK activation. Protein synthesis is strongly inhibited when AMPK is activated. It is concluded that amino acid-dependent signaling, a protein-anabolic signal, can be effectively antagonized by activation of AMPK.

【 授权许可】

Unknown