Single nucleotide polymorphisms in genes encoding penicillin-binding proteins in β-lactamase-negative ampicillin-resistant Haemophilus influenzae in Japan

BMC Research Notes

卷:11

Takashi Murakami¹  Runtuwene Lucky Ronald²  Yutaka Suzuki²  Norihito Tarumoto³  Shigefumi Maesaki³  Toshiyuki Yamaguchi³  Kazuhisa Misawa⁴  Kazuo Imai⁴  Akihiko Kawana⁴  Morichika Osa⁴  Yuji Kouzaki⁴  Takaaki Hamamoto⁵  Atsushi Yuki⁵  Takuya Maeda⁵  Shinsuke Tamura⁶ 

[1] Center for Clinical Infectious Diseases and Research, Saitama Medical University;

[2] Department of Computational Biology and Medical Science, Graduate School of Frontier Sciences, The University of Tokyo;

[3] Department of Infectious Disease and Infection Control, Saitama Medical University;

[4] Department of Infectious Diseases and Pulmonary Medicine, National Defense Medical College;

[5] Department of Laboratory Medicine, National Defense Medical College Hospital;

[6] Department of Pediatrics, National Defense Medical College;

关键词: Haemophilus influenzae;    β-Lactamase-negative ampicillin-resistant (BLNAR);    Penicillin binding protein;    SNP;   

DOI  :  10.1186/s13104-018-3169-0

来源: DOAJ

【 摘 要 】

Abstract Objective β-Lactamase-negative ampicillin-resistant Haemophilus influenzae is a common opportunistic pathogen of hospital- and community-acquired infections, harboring multiple single nucleotide polymorphisms in the ftsI gene, which codes for penicillin-binding protein-3. The objectives of this study were to perform comprehensive genetic analyses of whole regions of the penicillin-binding proteins in H. influenzae and to identify additional single nucleotide polymorphisms related to antibiotic resistance, especially to ampicillin and other cephalosporins. Results In this genome analysis of the ftsI gene in 27 strains of H. influenzae, 10 of 23 (43.5%) specimens of group III genotype β-lactamase-negative ampicillin-resistant H. influenzae were paradoxically classified as ampicillin-sensitive phenotypes. Unfortunately, we could not identify any novel mutations that were significantly associated with ampicillin minimum inhibitory concentrations in other regions of the penicillin-binding proteins, and we reconfirmed that susceptibility to β-lactam antibiotics was mainly defined by previously reported SNPs in the ftsI gene. We should also consider detailed changes in expression that lead to antibiotic resistance in the future because the acquisition of resistance to antimicrobials can be predicted by the expression levels of a small number of genes.

【 授权许可】

Unknown