Multidrug Resistant Klebsiella pneumoniae ST101 Clone Survival Chain From Inpatients to Hospital Effluent After Chlorine Treatment

Frontiers in Microbiology

卷:11

Anca Streinu-Cercel¹  Adrian Streinu-Cercel¹  Laura Ioana Popa²  Dan Oţelea⁴  Daniela Talapan⁴  Marius Surleac⁴  Simona Paraschiv⁴  Mihai Niţă⁵  Graţiela Grădişteanu Pîrcălăbioru⁶  Ilda Czobor Barbu⁶  Irina Gheorghe⁶  Marcela Popa⁶  Luminiţa Măruţescu⁶  Mariana Carmen Chifiriuc⁶ 

[1] Department II – Infectious Diseases, University of Medicine and Pharmacy “Carol Davila”, Bucharest, Romania;

[2] Department of Bioinformatics, National Institute of Research and Development for Biological Sciences, Bucharest, Romania;

[3] Department of Botany and Microbiology, Faculty of Biology, University of Bucharest, Bucharest, Romania;

[4] National Institute for Infectious Diseases “Matei Bals”, Bucharest, Romania;

[5] National Research and Development Institute for Industrial Ecology (ECOIND), Bucharest, Romania;

[6] Research Institute of the University of Bucharest, Bucharest, Romania;

关键词: wastewater treatment plant;    MDR Klebsiella pneumoniae;    whole-genome sequencing;    hospital wastewater chlorine treatment;    hospital sewage;   

DOI  :  10.3389/fmicb.2020.610296

来源: DOAJ

【 摘 要 】

In this paper we describe the transmission of a multi-drug resistant Klebsiella pneumoniae ST101 clone from hospital to wastewater and its persistence after chlorine treatment. Water samples from influents and effluents of the sewage tank of an infectious diseases hospital and clinical strains collected from the intra-hospital infections, during a period of 10 days prior to wastewater sampling were analyzed. Antibiotic resistant K. pneumoniae strains from wastewaters were recovered on selective media. Based on antibiotic susceptibility profiles and PCR analyses of antibiotic resistance (AR) genetic background, as well as whole-genome sequencing (Illumina MiSeq) and subsequent bioinformatic analyses, 11 ST101 K. pneumoniae strains isolated from hospital wastewater influent, wastewater effluent and clinical sector were identified as clonally related. The SNP and core genome analyses pointed out that five strains were found to be closely related (with ≤18 SNPs and identical cgMLST profile). The strains belonging to this clone harbored multiple acquired AR genes [blaCTX–M–15, blaOXA–48, blaOXA–1, blaSHV–106, blaTEM–150, aac(3)-IIa, aac(6′)-Ib-cr, oqxA10, oqxB17, fosA, catB3, dfrA14, tet(D)] and chromosomal mutations involved in AR (ΔmgrB, ΔompK35, amino acid substitutions in GyrA Ser83Tyr, Asp87Asn, ParC Ser80Tyr). Twenty-nine virulence genes involved in iron acquisition, biofilm and pili formation, adherence, and the type six secretion system – T6SS-III were identified. Our study proves the transmission of MDR K. pneumoniae from hospital to the hospital effluent and its persistence after the chlorine treatment, raising the risk of surface water contamination and further dissemination to different components of the trophic chain, including humans.

【 授权许可】

Unknown