The Effects of Non-Invasive Radiofrequency Treatment and Hyperthermia on Malignant and Nonmalignant Cells

International Journal of Environmental Research and Public Health

卷:11

Steven A. Curley¹  Flavio Palalon¹  Kelly E. Sanders¹  Nadezhda V. Koshkina¹ 

[1] Department of Surgical Oncology, Baylor College of Medicine, Houston, TX 77030, USA;

关键词: electromagnetic waves;    hyperthermia;    cancer;    autophagy;    mitochondria;    proliferation;   

DOI  :  10.3390/ijerph110909142

来源: DOAJ

【 摘 要 】

Background: Exposure of biological subjects to electromagnetic fieldswith a high frequency is associated with temperature elevation. In ourrecent studies, we reported that non-invasive radiofrequency (RF)treatment at 13.56 MHz with the field ranging from1 KeV to 20 KeV/m2 inhibits tumor progression in animals with abdominal tumor xenograftsand enhances the anticancer effect of chemotherapy. The RF treatment wasfollowed by temperature elevation in tumors to approximately 46 °Cduring 10 min of exposure. In contrast, the temperature of normaltissues remained within a normal range at approximately 37 °C. Whetherall biological effects of RF treatment are limited to its hyperthermicproperty remains unclear. Here, we compared how RF and hyperthermia (HT)treatments change the proliferation rate, oxygen consumption andautophagy in malignant and nonmalignant cells. Methods: In the currentstudy, cancer and nonmalignant cells of pancreatic origin were exposedto the RF field or to conventional HT at 46 °C, which was chosen basedon our previous in vivo studies of the tumor-specific RF-inducedhyperthermia. Results: Only RF treatment caused declines in cancer cellviability and proliferation. RF treatment also affected mitochondrialfunction in cancer cells more than HT treatment did and, unlike HTtreatment, was followed by the elevation of autophagosomes in thecytoplasm of cancer cells. Importantly, the effects of RF treatment werenegligible in nonmalignant cells. Conclusion: The obtained dataindicate that the effects of RF treatment are specific to cancer cellsand are not limited to itshyperthermic property.

【 授权许可】

Unknown