Lipid body formation during maturation of human mast cells

Journal of Lipid Research

卷:52

Sarah J. Butcher¹  Petri T. Kovanen¹  Reijo Käkelä²  Katarina Hattula³  Wolfgang J. Schneider³  Stefanie Schlager⁴  Jani Lappalainen⁴  Andrea Dichlberger⁴ 

[1] and;

[2] Department of Biosciences, FIN-00014 University of Helsinki, Finland and;

[3] Institute of Biotechnology, FIN-00014 University of Helsinki, Finland;

[4] Wihuri Research Institute, 00140 Helsinki, Finland;

关键词: arachidonic acid;    eicosanoids;    fatty acid;    inflammation;    perilipin;    secretory granules;   

DOI  :  

来源: DOAJ

【 摘 要 】

Lipid droplets, also called lipid bodies (LB) in inflammatory cells, are important cytoplasmic organelles. However, little is known about the molecular characteristics and functions of LBs in human mast cells (MC). Here, we have analyzed the genesis and components of LBs during differentiation of human peripheral blood-derived CD34+ progenitors into connective tissue-type MCs. In our serum-free culture system, the maturing MCs, derived from 18 different donors, invariably developed triacylglycerol (TG)-rich LBs. Not known heretofore, the MCs transcribe the genes for perilipins (PLIN)1-4, but not PLIN5, and PLIN2 and PLIN3 display different degrees of LB association. Upon MC activation and ensuing degranulation, the LBs were not cosecreted with the cytoplasmic secretory granules. Exogenous arachidonic acid (AA) enhanced LB genesis in Triacsin C-sensitive fashion, and it was found to be preferentially incorporated into the TGs of LBs. The large TG-associated pool of AA in LBs likely is a major precursor for eicosanoid production by MCs. In summary, we demonstrate that cultured human MCs derived from CD34+ progenitors in peripheral blood provide a new tool to study regulatory mechanisms involving LB functions, with particular emphasis on AA metabolism, eicosanoid biosynthesis, and subsequent release of proinflammatory lipid mediators from these cells.

【 授权许可】

Unknown