Antibody levels to recombinant VAR2CSA domains vary with Plasmodium falciparum parasitaemia, gestational age, and gravidity, but do not predict pregnancy outcomes

Malaria Journal

卷:17

Bruce Swihart¹  Sunthorn Pond-Tor²  Jonathan D. Kurtis²  Robert Morrison³  Michal Fried³  Naissem Andemel³  David L. Narum³  Patrick E. Duffy³  Oumar Attaher⁴  Adama B. Dembele⁴  Alassane Dicko⁴  Youssoufa Sidibe⁴  Almahamoudou Mahamar⁴  Moussa B. Kanoute⁴  Kadidia B. Cisse⁴  Sekouba Keita⁴  Bacary S. Diarra⁴  Amadou Barry⁴ 

[1] Biostatistics Research Branch, National Institute of Allergy and Infectious Diseases, NIH;

[2] Center for International Health Research, Rhode Island Hospital, Brown University Medical School;

[3] Laboratory of Malaria Immunology and Vaccinology, National Institute of Allergy and Infectious Diseases, NIH;

[4] Malaria Research & Training Center, Faculty of Medicine, Pharmacy and Dentistry, University of Sciences Techniques and Technologies of Bamako;

关键词: Placental malaria;    VAR2CSA;    Birth weight;    Pregnancy loss;    Preterm delivery;    Anaemia;   

DOI  :  10.1186/s12936-018-2258-9

来源: DOAJ

【 摘 要 】

Abstract Background Maternal malaria is a tropical scourge associated with poor pregnancy outcomes. Women become resistant to Plasmodium falciparum pregnancy malaria as they acquire antibodies to the variant surface antigen VAR2CSA, a leading vaccine candidate. Because malaria infection may increase VAR2CSA antibody levels and thereby confound analyses of immune protection, gravidity-dependent changes in antibody levels during and after infection, and the effect of VAR2CSA antibodies on pregnancy outcomes were evaluated. Methods Pregnant women enrolled in a longitudinal cohort study of mother-infant pairs in Ouelessebougou, Mali provided plasma samples at enrollment, gestational week 30–32, and delivery. Antibody levels to VAR2CSA domains were measured using a multiplex bead-based assay. Results Antibody levels to VAR2CSA were higher in multigravidae than primigravidae. Malaria infection was associated with increased antibody levels to VAR2CSA domains. In primigravidae but not in secundigravidae or multigravidae, antibodies levels sharply declined after an infection. A relationship between any VAR2CSA antibody specificity and protection from adverse pregnancy outcomes was not detected. Conclusions During malaria infection, primigravidae acquire short-lived antibodies. The lack of an association between VAR2CSA domain antibody reactivity and improved pregnancy outcomes suggests that the recombinant proteins may not present native epitopes targeted by protective antibodies.

【 授权许可】

Unknown