Screening of Bacterial Endophytes Able to Promote Plant Growth and Increase Salinity Tolerance

Applied Sciences

卷:10

Madel Carmen Orozco-Mosqueda¹  BernardR. Glick¹  Nicoletta Favale²  Elisa Bona³  Elisa Gamalero⁴  Guido Lingua⁴  Patrizia Cesaro⁴  Graziella Berta⁴  Giorgia Novello⁴  Nadia Massa⁴ 

[1] Department of Biology, University of Waterloo, Waterloo, ON N2L 3G1, Canada;

[2] Department of Life Sciences and Biotechnologies, University of Ferrara UNIFE, 44121 Ferrara, Italy;

[3] Dipartimento di Scienze e Innovazione Tecnologica, Università del Piemonte Orientale, Piazza San Eusebio 5, 13100 Vercelli, Italy;

[4] Dipartimento di Scienze e Innovazione Tecnologica, Università del Piemonte Orientale, Viale T. Michel 11, 15121 Alessandria, Italy;

关键词: endophyte isolation;    physiological activities;    plant growth promotion;    salt stress;    ACC deaminase;   

DOI  :  10.3390/app10175767

来源: DOAJ

【 摘 要 】

Bacterial endophytes can colonize plant tissues without harming the plant. Instead, they are often able to increase plant growth and tolerance to environmental stresses. In this work, new strains of bacterial endophytes were isolated from three economically important crop plants (sorghum, cucumber and tomato) grown in three different regions in soils with different management. All bacterial strains were identified by 16S rRNA sequencing and characterized for plant beneficial traits. Based on physiological activities, we selected eight strains that were further tested for their antibiotic resistance profile and for the ability to efficiently colonize the interior of sorghum plants. According to the results of the re-inoculation test, five strains were used to inoculate sorghum seeds. Then, plant growth promotion activity was assessed on sorghum plants exposed to salinity stress. Only two bacterial endophytes increased plant biomass, but three of them delayed or reduced plant salinity stress symptoms. These five strains were then characterized for the ability to produce the enzyme 1-aminocyclopropane-1-carboxylate (ACC) deaminase, which is involved in the increase of stress tolerance. Pseudomonas brassicacearum SVB6R1 was the only strain that was able to produce this enzyme, suggesting that ACC deaminase is not the only physiological trait involved in conferring plant tolerance to salt stress in these bacterial strains.

【 授权许可】

Unknown