Metabolomics and Marine Biotechnology: Coupling Metabolite Profiling and Organism Biology for the Discovery of New Compounds

Frontiers in Marine Science

卷:7

Peter J. Schupp¹  Sven Rohde²  Miriam Reverter²  Nathalie Tapissier-Bontemps³  Christelle Parchemin⁴ 

[1] Helmholtz Institute for Functional Marine Biodiversity at the University of Oldenburg (HIFMB), Oldenburg, Germany;

[2] Institute for Chemistry and Biology of the Marine Environment (ICBM) at the University of Oldenburg, Wilhelmshaven, Germany;

[3] Labex Corail, Moorea, French Polynesia;

[4] PSL Research University, USR EPHE-UPVD-CNRS 3278, CRIOBE, Université de Perpignan, Perpignan, France;

关键词: metabolomics;    marine biotechnology;    marine natural products;    chemical ecology;    omics integration;   

DOI  :  10.3389/fmars.2020.613471

来源: DOAJ

【 摘 要 】

The high diversity of marine natural products represents promising opportunities for drug discovery, an important area in marine biotechnology. Within this context, high-throughput techniques such as metabolomics are extremely useful in unveiling unexplored chemical diversity at much faster rates than classical bioassay-guided approaches. Metabolomics approaches enable studying large sets of metabolites, even if they are produced at low concentrations. Although, metabolite identification remains the main metabolomics bottleneck, bioinformatic tools such as molecular networks can lead to the annotation of unknown metabolites and discovery of new compounds. A metabolomic approach in drug discovery has two major advantages: it enables analyses of multiple samples, allowing fast dereplication of already known compounds and provides a unique opportunity to relate metabolite profiles to organisms’ biology. Understanding the ecological and biological factors behind a certain metabolite production can be extremely useful in enhancing compound yields, optimizing compound extraction or in selecting bioactive compounds. Metazoan-associated microbiota are often responsible for metabolite synthesis, however, classical approaches only allow studying metabolites produced from cultivatable microbiota, which often differ from the compounds produced within the host. Therefore, coupling holobiome metabolomics with microbiome analysis can bring new insights to the role of microbiota in compound production. The ultimate potential of metabolomics is its coupling with other “omics” (i.e., transcriptomics and metagenomics). Although, such approaches are still challenging, especially in non-model species where genomes have not been annotated, this innovative approach is extremely valuable in elucidating gene clusters associated with biosynthetic pathways and will certainly become increasingly important in marine drug discovery.

【 授权许可】

Unknown