Oral Mesenchymal Stromal Cells in Systemic Sclerosis: Characterization and Response to a Hyaluronic-Acid-Based Biomaterial

Applied Sciences

卷:11

Adriana Elena Bulboacă¹  Ștefan Ioan Stratul²  Alexandra Roman³  Iulia Cristina Micu³  Alina Stanomir³  Andrada Soancă³  Emoke Pall⁴  Carmen Mihaela Mihu⁵  Cristina Pamfil⁶  Simona Rednic⁶  Aurel Popa-Wagner⁷ 

[1] Department of Pathophysiology, Iuliu Haţieganu University of Medicine and Pharmacy Cluj-Napoca, Victor Babes St., No. 2-4, 400012 Cluj-Napoca, Romania;

[2] Department of Periodontology, Anton Sculean Center for Research of Periodontal and Periimplant Diseases, Faculty of Dental Medicine, Victor Babes University of Medicine and Pharmacy, Bulevardul Revolutiei din 1989, No. 9, 300230 Timisoara, Romania;

[3] Department of Periodontology, Faculty of Dental Medicine, Iuliu Haţieganu University of Medicine and Pharmacy Cluj-Napoca, Victor Babeş St., No. 15, 400012 Cluj-Napoca, Romania;

[4] Department of Veterinary Reproduction, Obstetrics and Gynaecology, Faculty of Veterinary Medicine, University of Agricultural Sciences and Veterinary Medicine, Mănăștur St., No. 3-5, 400372 Cluj-Napoca, Romania;

[5] Discipline of Histology, Department of Morphological Sciences, Iuliu Haţieganu University of Medicine and Pharmacy Cluj-Napoca, Louis Pasteur St., No. 6, 400349 Cluj-Napoca, Romania;

[6] Discipline of Rheumatology, Iuliu Haţieganu University of Medicine and Pharmacy Cluj-Napoca, Clinicilor St., No. 2, 400000 Cluj-Napoca, Romania;

[7] Research Center for Normal and Pathological Aging, ARES, University of Medicine and Pharmacy of Craiova, Petru Rareș Str., No. 2-4, 200349 Craiova, Romania;

关键词: mesenchymal stromal cell;    surface antigens;    differentiation;    systemic sclerosis;    hyaluronic acid;   

DOI  :  10.3390/app11178101

来源: DOAJ

【 摘 要 】

Introduction. As oral mesenchymal stromal cells (MSCs) have not, to date, been isolated from systemic sclerosis (SSc) patients, the aim of this in vitro experiment was to characterize gingival MSCs (SScgMSCs) and granulation tissue MSCs (SScgtMSCs) from SSc and to evaluate their functionality in comparison to healthy MSCs (hMSCs), in normal or hyaluronic acid (HA) culture media. Materials and Methods. Isolated cells were described by immunophenotyping of surface antigen make-up and by trilineage mesenchymal differentiation capacity. Colony-Forming Unit-Fibroblast (CFU-F) test and migration potential evaluated MSC functionality. Results. All types of MSCs displayed positivity for the following surface markers: CD29, CD73, CD90, CD105, CD44, and CD79a. These cells did not express CD34, CD45, HL-DR, and CD14. Isolated MSCs differentiated into osteoblasts, adipocytes, and chondroblasts. The frequency of CFU-F for SScgtMSCs was significantly lower than that of hMSCs (p = 0.05) and SScgMSCs (p = 0.004) in normal medium, and also markedly lower than that of SScgMSCs (p = 0.09) in HA medium. Following HA exposure, both SScgMSCs and SScgtMSCs migrated significantly less (p = 0.033 and p = 0.005, respectively) than hMSCs. Conclusions. A reduced functionality of MSCs derived from SSc as compared to hMSCs was observed. HA in culture medium appeared to significantly stimulate the migration potential of hMSCs.

【 授权许可】

Unknown