Gene Expression Changes after Parental Exposure to Metals in the Sea Urchin Affect Timing of Genetic Programme of Embryo Development

Biology

卷:10

Salvatore Costa¹  MariaAntonietta Ragusa¹  Girolama Biondo²  Marcello Tagliavia³  Aldo Nicosia³  Angela Cuttitta⁴  CarmeloDaniele Bennici⁴  MarilenaDi Natale⁴  Tiziana Masullo⁴  Marianna Musco⁴ 

[1] Department of Biological, Chemical and Pharmaceutical Sciences and Technologies (STEBICEF), University of Palermo, Viale delle Scienze Ed. 16, 90128 Palermo, Italy;

[2] Institute for Anthropic Impacts and Sustainability in Marine Environment-National Research Council (IAS-CNR), Detached Unit of Capo Granitola, Via del mare 3, 91021 Campobello di Mazara, Italy;

[3] Institute for Biomedical Research and Innovation-National Research Council-(IRIB-CNR), Via Ugo La Malfa 153, 90146 Palermo, Italy;

[4] Institute for Studies on the Mediterranean-National Research Council (ISMED-CNR), Detached Unit of Palermo, Via Filippo Parlatore 65, 90145 Palermo, Italy;

关键词: sea urchin;    redox homeostasis;    parental exposure;    intergenerational effects;    embryo development;    gene expression profiles;   

DOI  :  10.3390/biology10020103

来源: DOAJ

【 摘 要 】

It is widely accepted that phenotypic traits can be modulated at the epigenetic level so that some conditions can affect the progeny of exposed individuals. To assess if the exposure of adult animals could result in effects on the offspring, the Mediterranean sea urchin and its well-characterized gene regulatory networks (GRNs) was chosen as a model. Adult animals were exposed to known concentrations of zinc and cadmium (both individually and in combination) for 10 days, and the resulting embryos were followed during the development. The oxidative stress occurring in parental gonads, embryo phenotypes and mortality, and the expression level of a set of selected genes, including members of the skeletogenic and endodermal GRNs, were evaluated. Increased oxidative stress at F₀, high rates of developmental aberration with impaired gastrulation, in association to deregulation of genes involved in skeletogenesis (dri, hex, sm50, p16, p19, msp130), endodermal specification (foxa, hox11/13b, wnt8) and epigenetic regulation (kat2A, hdac1, ehmt2, phf8 and UBE2a) occurred either at 24 or 48 hpf. Results strongly indicate that exposure to environmental pollutants can affect not only directly challenged animals but also their progeny (at least F₁), influencing optimal timing of genetic programme of embryo development, resulting in an overall impairment of developmental success.

【 授权许可】

Unknown