Stabilization of HIF-1α in Human Retinal Endothelial Cells Modulates Expression of miRNAs and Proangiogenic Growth Factors

Frontiers in Pharmacology

卷:11

Claudio Bucolo¹  Filippo Drago¹  Chiara Bianca Maria Platania²  Francesca Lazzara²  Maria Consiglia Trotta³  Michele D’Amico³  Marilena Galdiero³  Francesco Petrillo³  Settimio Rossi⁴  Carlo Gesualdo⁴ 

[1] Center for Research in Ocular Pharmacology-CERFO, University of Catania, Catania, Italy;

[2] Department of Biomedical and Biotechnological Sciences, School of Medicine, University of Catania, Catania, Italy;

[3] Department of Experimental Medicine, Division of Pharmacology, University of Campania “Luigi Vanvitelli”, Naples, Italy;

[4] Eye Clinic, Multidisciplinary Department of Medical, Surgical and Dental Sciences, University of Campania “Luigi Vanvitelli”, Naples, Italy;

关键词: hypoxia-inducible-factor-1α;    vascular endothelial growth factor;    transforming growth factor beta;    retina;    diabetic retinopathy;    inflammation;   

DOI  :  10.3389/fphar.2020.01063

来源: DOAJ

【 摘 要 】

Retinal hypoxia is one of the causative factors of diabetic retinopathy and is also one of the triggers of VEGF release. We hypothesized that specific dysregulated miRNAs in diabetic retinopathy could be linked to hypoxia-induced damage in human retinal endothelial cells (HRECs). We investigated in HRECs the effects of chemical (CoCl2) hypoxia on the expression of HIF-1α, VEGF, PlGF, and of a focused set of miRNAs. We found that miR-20a-5p, miR-20b-5p, miR-27a-3p, miR-27b-3p, miR-206-3p, miR-381-3p correlated also with expression of TGFβ signaling pathway genes in HRECs, challenged with chemical hypoxic stimuli. In conclusion, our data suggest that retinal angiogenesis would be promoted, at least under HIF-1α activation, by upregulation of PlGF and other factors such as miRNAs, VEGFA, and TGFβ1.

【 授权许可】

Unknown