Production of the Allergenic Protein Alt a 1 by Alternaria Isolates from Working Environments

International Journal of Environmental Research and Public Health

卷:12

Justyna Skóra¹  Anna Otlewska¹  Beata Gutarowska¹  Joanna Leszczyńska²  Iwona Majak²  Łukasz Stępień³ 

[1] Institute of Fermentation Technology and Microbiology, Lodz University of Technology,171/173 Wólczańska Str, 90-924 Łódź, Poland;

[2] Institute of General Food Chemistry, Lodz University of Technology, 171/173 Wólczańska Str,90-924 Łódź, Poland;

[3] Institute of Plant Genetics, Polish Academy of Sciences, 34 Strzeszyńska Str, 60-479 Poznań, Poland;

关键词: Alternaria;    workplaces;    Alt a 1 protein;    Alt a 1 gene;    ELISA;   

DOI  :  10.3390/ijerph120202164

来源: DOAJ

【 摘 要 】

The aim of the study was to evaluate the ability of Alternaria isolates from workplaces to produce Alt a 1 allergenic protein, and to analyze whether technical materials (cellulose, compost, leather) present within the working environment stimulate or inhibitAlt a 1 production (ELISA test). Studies included identification of the isolated molds by nucleotide sequences analyzing of the ITS1/ITS2 regions, actin, calmodulin and Alt a 1 genes. It has been shown that Alternaria molds are significant part of microbiocenosis in the archive, museum, library, composting plant and tannery (14%–16% frequency in the air). The presence of the gene encoding the Alt a 1 protein has been detected for the strains: Alternaria alternata, A. lini, A. limoniasperae A. nobilis and A. tenuissima. Environmental strains produced Alt a 1 at higher concentrations (1.103–6.528 ng/mL) than a ATCC strain (0.551–0.975 ng/mL). It has been shown that the homogenization of the mycelium and the use of ultrafiltration allow a considerable increase of Alt a 1 concentration. Variations in the production of Alt a 1 protein, depend on the strain and extraction methods. These studies revealed no impact of the technical material from the workplaces on the production ofAlt a 1 protein.

【 授权许可】

Unknown