The stimulating effects of polyphenol and protein fractions from jelly fig (Ficus awkeotsang Makino) achenes against proliferation of leukemia cells

Journal of Food and Drug Analysis

卷:25

Chii-Ming Jiang¹  Ming-Chang Wu²  Yi-Zhen Shih²  Ai-Jun Huang³  Chih-Yao Hou⁴ 

[1] Department of Food Science and Technology, National Taitung Junior College, Taitung, Taiwan;

[2] Department of Food Science, National Pingtung University of Science and Technology, Pingtung, Taiwan;

[3] Department of Leisure, Recreation and Tourism Management, Tzu Hui Institute of Technology, Pingtung, Taiwan;

[4] Department of Seafood Science, National Kaohsiung Marine University, Kaohsiung City, Taiwan;

关键词: antiproliferative;    immunomodulatory;    jelly fig;    U937 cells;   

DOI  :  10.1016/j.jfda.2016.10.015

来源: DOAJ

【 摘 要 】

This study aimed to investigate the direct and immune-stimulated antiproliferative activities of jelly fig achenes fractions including pectinesterase inhibitors, crude polyphenols extract, and purified polyphenols extract (PP). Beside the measurement of cell viability of U937, the quantity of cytokines in conditioned medium and morphologic changes in leukemia were observed. After surveying all fractions in jelly fig, the obtained fractions of polyphenol exhibited the highest stimulating effects and directly cytotoxic effects against leukemia with the lowest effect found in protein fractions. The leukemia treated by our PP fraction showed dose-dependent response between the concentration and G2/M cell numbers of the U937 cells. The PP fraction had more pronounced effect on immune-stimulated than direct antiproliferative activities. The finding was also supported by morphological analysis by showing the formation of apoptotic bodies and differentiation from immature U937 cells into mature monocytes/macrophages on cells cultured with PP-conditioned medium. In conclusion, polyphenol fraction of pectinesterase inhibitors from jelly fig showed the immune-stimulated antiproliferative activities against U937 cell.

【 授权许可】

Unknown