Immunomodulatory Effects of Liriope Platyphylla Water Extract on Lipopolysaccharide-Activated Mouse Macrophage

Nutrients

Hye Kyung Kim¹  Ji Young Lee¹  Hyo-Sang Han³  Young-Jin Kim¹  Hyun Joo Kim¹  Yoon-Sang Kim¹  Hyung Min Kim²  Seong-Gyu Ko²  Hyo-Jin An⁴  Young-Jong Lee¹ 

[1]College of Korean Medicine, Gachon University, Seongnam 461-701, Korea

[2]College of Oriental Medicine, Kyung Hee University, Seoul 130-701, Korea

[3]College of Social Science, Joongbu University, Geumsan 312-702, Korea

[4]College of Oriental Medicine, Sangji University, Wonju 220-702, Korea

关键词: Liriope platyphylla;    immunomodulatory;    macrophage;    cytokine;    nitric oxide;    transcription factor;   

DOI  :  10.3390/nu4121887

来源: mdpi

【 摘 要 】

The tuber of Liriope platyphylla Wang et Tang (Liliaceae), also known as Liriopis tuber, is famous in Oriental medicine owing to its tonic, antitussive, expectorant and anti-asthmatic properties. In the present study, the effects of Liriopis tuber water extract (LP) on proinflammatory mediators secreted from lipopolysaccharide (LPS)-induced cultured RAW 264.7 mouse macrophages were investigated. Nitric oxide (NO), prostaglandin E2 (PGE2) and intracellular calcium release were measured after 24 h incubation. Various cytokines and nuclear transcription factors (NF-κB and CREB) of LPS-induced RAW 264.7 were measured by a multiplex bead array assay based on xMAP technology. LP (up to 200 μg/mL) significantly decreased levels of nitric oxide (NO), interleukin (IL)-6, IL-10, IL-12p40, interferon-inducible protein-10, keratinocyte-derived chemokine, monocyte chemotactic protein-1, vascular endothelial growth factor, granulocyte macrophage-colony stimulating factor, platelet derived growth factor, PGE2, intracellular calcium, NF-κB and CREB in LPS-induced RAW 264.7 cells (p < 0.05). The results suggest that LP has immunomodulatory activity to reduce excessive immune reactions during the activation of macrophages by LPS. Further studies are needed to verify the precise mechanism regulating immunomodulatory activities of LP.

【 授权许可】

CC BY   
© 2012 by the authors; licensee MDPI, Basel, Switzerland.