Effect of Cigarette Smoking on Selected Antioxidant Enzymes and Oxidative Stress Biomarkers

Journal of Clinical and Diagnostic Research

卷:14

Sangeeta Singh¹  Preeti Sharma²  Tapan Mohapatra²  Basant Joshi³  Pradeep Kumar⁴ 

[1] Associate Professor, Department of Biochemistry, Government Medical College, Haldwani, Uttarakhand, India.;

[2] Associate Professor, Department of Biochemistry, Santosh Medical College, Ghaziabad, Uttar Pradesh, India.;

[3] PhD Scholar, Department of Biochemistry, Santosh Medical College, Ghaziabad, Uttar Pradesh, India.;

[4] Professor, Department of Biochemistry, Santosh Medical College, Ghaziabad, Uttar Pradesh, India.;

关键词: catalase;    cigarette smokers;    glutathione peroxidase;    serum oxidase dismutase;   

DOI  :  10.7860/JCDR/2020/45948.14138

来源: DOAJ

【 摘 要 】

Introduction: Cigarette Smoking (CS) is the single greatestpreventable cause of disease and death and is rich in ReactiveOxygen and Nitrogen Species (ROS and RNS). These can causethe production of other free radicals, which, in turn, initiate lipidperoxidation and cause several diseases. Free radical scavengerenzymes namely Superoxide Dismutase (SOD), Catalase (CAT)and Glutathione Peroxidase (GPx) represent the enzymatic partthat have the ability to inhibit oxidative stress by scavenging thehighly destructive free radicals.Aim: To study the effect of CS on selected antioxidant enzymesand oxidative stress biomarkers.Materials and Methods: A case control study was conductedfrom September 2016 to September 2019 in which total of 284healthy (without any systemic diseases) cigarette smokers (cases)in the age group of 18-60 years compared with age and sexmatched 284 nonsmokers (controls) were included in the study.Estimation of serum 8-hydroxydeoxyguanosine (8-OHdG) byEnzyme Linked Immunosorbant Assay (ELISA), Malondialdehyde(MDA) by Thiobarbuturic Acid Reactive Substances (TBARS), SODby water soluble tetrazolium salt 1, GPx and CAT by colorimetricmethod. The analysis was carried out using the SPSS 19.0.2program for windows. Unpaired t-test and one-way ANOVA wereused to analyse all the data for statistical significance.Results: The mean Serum MDA and 8-OHdG levels weresignificantly raised 7.47±1.84, 63.41±22.44 as compared tononsmokers (3.90±1.03, 40.04±20.14) and serum SOD, Gpxand CAT levels were decreased 62.55±19.97, 44.45±16.60 and12.92±10.16 in cigarette smokers as compared to nonsmokers274.04±68.37, 208.56±75.63 and 127.82±18.68, respectively.These differences were also found to be statistically significantin cigarette smokers according to duration and number ofcigarette smoked at the level of <0.05.Conclusion: Cigarette Smoking, especially long-term smokingmay leads to significant changes in the enzymatic antioxidantdefense systems of smokers. Discontinuation of smoking andgeneral awareness needs to be created to minimise the risk ofsmoking related diseases.

【 授权许可】

Unknown