Preserved Cerebral Oxygen Metabolism in Astrocytic Dysfunction: A Combination Study of 15O-Gas PET with 14C-Acetate Autoradiography

Brain Sciences

卷:9

Eku Shimosegawa¹  Yasukazu Kanai¹  Tadashi Watabe²  Genki Horitsugi²  CarlaMari Macaisa²  Victor Romanov²  Yuwei Liu²  Jun Hatazawa²  Hiroki Kato² 

[1] Department of Molecular Imaging in Medicine, Osaka University Graduate School of Medicine,Suita 565-0871, Japan;

[2] Department of Nuclear Medicine and Tracer Kinetics, Osaka University Graduate School of Medicine, Suita 565-0871, Japan;

关键词: fluorocitrate;    astrocyte;    oxygen consumption;    TCA cycle;    ¹⁵O-gas PET;    cerebral blood flow;    cerebral metabolic rate of oxygen;    ¹⁴C-acetate;   

DOI  :  10.3390/brainsci9050101

来源: DOAJ

【 摘 要 】

Fluorocitrate (FC) is a specific metabolic inhibitor of the tricarboxylic acid (TCA) cycle in astrocytes. The purpose of this study was to evaluate whether inhibition of the astrocyte TCA cycle by FC would affect the oxygen metabolism in the rat brain. At 4 h after the intracranial FC injection, the rats (n = 9) were investigated by ¹⁵O-labeled gas PET to measure the cerebral blood flow (CBF), the cerebral metabolic rate of oxygen (CMRO₂), oxygen extraction fraction (OEF), and cerebral blood volume (CBV). After the ¹⁵O-gas PET, the rats were given an intravenous injection of ¹⁴C-acetate for autoradiography. ¹⁵O-gas PET showed no significant differences in any of the measured parameters between the ipsilateral and contralateral striatum (high dose group: CBF (54.4 ± 8.8 and 55.3 ± 11.6 mL/100 mL/min), CMRO₂ (7.0 ± 0.9 and 7.1 ± 1.2 mL/100 mL/min), OEF (72.0 ± 8.9 and 70.8 ± 8.2%), and CBV (4.1 ± 0.8 and 4.2 ± 0.9 mL/100 mL), respectively). In contrast, the ¹⁴C-acetate autoradiography revealed a significant inhibition of the astrocyte metabolism in the ipsilateral striatum. The regional cerebral oxygen consumption as well as the hemodynamic parameters were maintained even in the face of inhibition of the astrocyte TCA cycle metabolism in the rat brain.

【 授权许可】

Unknown