Expression of PEP carboxylase from Escherichia coli complements the phenotypic effects of pyruvate carboxylase mutations in Saccharomyces cerevisiae

FEBS Letters

Flores, Carmen-Lisset¹  Gancedo, Carlos¹ 

[1] Instituto de Investigaciones Biomédicas, Unidad de Bioquı́mica y Genética de Levaduras, Arturo Duperier 4, E-28029 Madrid, Spain

关键词: PEP carboxylase;    Pyruvate carboxylase;    TCA cycle;    Glucose metabolism (yeast);   

DOI  :  10.1016/S0014-5793(97)00854-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We investigated the effects of the expression of the Escherichia coli ppc gene encoding PEP carboxylase in Saccharomyces cerevisiae mutants devoid of pyruvate carboxylase. Functional expression of the ppc gene restored the ability of the yeast mutants to grow in glucose-ammonium medium. Growth yield in this medium was the same in the transformed yeast than in the wild type although the growth rate of the transformed yeast was slower. Growth in pyruvate was slowed down in the transformed strain, likely due to a futile cycle produced by the simultaneous action of PEP carboxykinase and PEP carboxylase.

【 授权许可】

Unknown