Lessened malate inhibition of guard‐cell phosphoenolpyruvate carboxylase velocity during stomatal opening

FEBS Letters

Zhang, Shu Qiu²  Chollet, Raymond¹  Outlaw, William H² 

[1] Department of Biochemistry, University of Nebraska at Lincoln, East Campus, Lincoln, NE 68583-0718, USA;Department of Biological Science, Florida State University, Tallahassee, FL 32306-3050, USA

关键词: Guard cell;    l-Malate;    Phosphoenolpyruvate;    Stoma;    Vicia faba;    CAM;    crassulacean acid metabolism;    PEP;    phosphoenolpyruvate;    PEPC;    PEP carboxylase;   

DOI  :  10.1016/0014-5793(94)00916-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Leaflets of Vicia faba with closed stomata or with opening stomata were freeze-dried. Excised guard-cell pairs were assayed individually under suboptimal conditions (pH 7.1 and subsaturating substrate) for phosphoenolpyruvate carboxylase (PEPC; EC 4.1.1.31) using quantitative histochemical procedures. l-Malate, 400 μM, significantly inhibited guard-cell PEPC activity of closed stomata but not that of opening stomata. We postulate that the lessened sensitivity of guard-cell PEPC activity to malate inhibition is an important regulatory feature of stomatal opening, which is associated with malate accumulation.

【 授权许可】

Unknown