Lethal Outcome of Leptospirosis in Southern Russia: Characterization of Leptospira Interrogans Isolated from a Deсeased Teenager

International Journal of Environmental Research and Public Health

卷:17

AlexeyU. Goncharov¹  AnnaR. Litovko¹  Kamil Khafizov²  Birgitta Evengard³  EvgeniyV. Kovalev⁴  ElenaB. Zueva⁵  YuliaA. Panferova⁵  NataliyaA. Stoyanova⁵  VladimirG. Dedkov⁵  DianaE. Valutite⁵  NikolaiK. Tokarevich⁵  ElenaV. Zueva⁵  AlexandrV. Semenov⁵  AndreiE. Samoilov⁵  YuliaV. Ostankova⁵ 

[1] Center for Hygiene and Epidemiology in the Rostov Region, Federal Service on Consumers’ Rights Protection and Human Well-Being Surveillance, 344029 Rostov-on-Don, Russia;

[2] Central Research Institute for Epidemiology, Federal Service on Consumers’ Rights Protection and Human Well-Being Surveillance, 111123 Moscow, Russia;

[3] Department of Clinical Microbiology, Umeå University, 90187 Umeå, Sweden;

[4] Department of Federal Service on Consumers’ Rights Protection and Human Well-Being Surveillance in the Rostov Region, 344019 Rostov-on-Don, Russia;

[5] Pasteur Institute, Federal Service on Consumers’ Rights Protection and Human Well-Being Surveillance, 197101 Saint-Petersburg, Russia;

关键词: Leptospira;    MALDI-TOF MS;    PCR;    high-throughput sequencing;    MAT;    Russia;   

DOI  :  10.3390/ijerph17124238

来源: DOAJ

【 摘 要 】

This article describes a lethal case of leptospirosis that occurred in Southern Russia. The Leptospira strain was isolated and characterized using a microscopic agglutination test, MALDI-TOF mass spectrometry, targeted PCR, and high-throughput sequencing. We show that molecular and mass-spectrometry methods can be an alternative to conventional methods of leptospirosis diagnostics and Leptospira study, which require highly qualified staff and can be performed only at specialized laboratories. We also report the first whole genome of L. interrogans isolated in Russia.

【 授权许可】

Unknown