In-Situ Electrophoretic Mobility Determination by Particle Image Velocimetry for Efficient Microfluidic Enrichment of Bacteria

Proceedings

卷:1

Ole Behrmann¹  Richard Rietzel¹  Matthias Hügle¹  Gerald A. Urban¹  Gregory Dame² 

[1] IMTEK—Department of Microsystems Engineering, University of Freiburg, 79085 Freiburg, Germany;

[2] Institute for Microbiology and Virology, Brandenburg Medical School, 16816 Neuruppin, Germany;

关键词: bacteria enrichment;    microfluidics;    free-flow electrophoresis (FFE);    Particle Image Velocimetry (PIV);    electrophoretic mobility;    quantitative polymerase chain reaction (qPCR);   

DOI  :  10.3390/proceedings1040535

来源: DOAJ

【 摘 要 】

We present a novel approach for the efficiency enhancement of microfluidic bacteria enrichment systems based on free-flow electrophoresis (FFE). FFE efficiency is highly dependent on the electrophoretic mobility μ of the bacteria. As μ varies strongly with the suspension medium, fast and accurate determination of μ is needed to achieve optimal enrichment performance from different suspension media. For the first time, μ is determined in-situ for multiple media during on-chip FFE by Particle Image Velocimetry (PIV) of fluorescent bacteria, obviating the need for separate measurement equipment or chemical staining of the bacteria.

【 授权许可】

Unknown