Retrieval of the Complete Coding Sequence of the UK-Endemic Tatenale Orthohantavirus Reveals Extensive Strain Variation and Supports Its Classification as a Novel Species

Viruses

卷:12

Ian Ashpole¹  Gabby Drake¹  William Edema²  JosephG. Chappell²  JonathanK. Ball²  Okechukwu Onianwa²  Frederick Kumi-Ansah²  C.Patrick McClure²  Theocharis Tsoleridis²  Malcolm Bennett³  RachaelE. Tarlinton³  Phillipa Dobbs⁴ 

[1] Chester Zoo, Chester, Cheshire CH2 1EU, UK;

[2] School of Life Sciences, University of Nottingham, Nottingham NG7 2UH, UK;

[3] School of Veterinary Science, University of Nottingham, Sutton Bonnington, Loughborough LE12 5RD, UK;

[4] Twycross Zoo, Atherstone, Warwickshire CV9 3PX, UK;

关键词: Orthohantavirus;    hantavirus;    high-throughput sequencing;    virus discovery;    field vole;    United Kingdom;   

DOI  :  10.3390/v12040454

来源: DOAJ

【 摘 要 】

Orthohantaviruses are globally distributed viruses, associated with rodents and other small mammals. However, data on the circulation of orthohantaviruses within the UK, particularly the UK-endemic Tatenale virus, is sparse. In this study, 531 animals from five rodent species were collected from two locations in northern and central England and screened using a degenerate, pan- orthohantavirus RT-PCR assay. Tatenale virus was detected in a single field vole (Microtus agrestis) from central England and twelve field voles from northern England. Unbiased high-throughput sequencing of the central English strain resulted in the recovery of the complete coding sequence of a novel strain of Tatenale virus, whilst PCR-primer walking of the northern English strain recovered almost complete coding sequence of a previously identified strain. These findings represented the detection of a third lineage of Tatenale virus in the United Kingdom and extended the known geographic distribution of these viruses from northern to central England. Furthermore, the recovery of the complete coding sequence revealed that Tatenale virus was sufficiently related to the recently identified Traemersee virus, to meet the accepted criteria for classification as a single species of orthohantavirus.

【 授权许可】

Unknown