Identification of a Novel Human Rhinovirus C Type by Antibody Capture VIDISCA-454

Viruses

Seyed Mohammad Jazaeri Farsani¹  Bas B. Oude Munnink¹  Marta Canuti¹  Martin Deijs¹  Matthew Cotten²  Maarten F. Jebbink¹  Joost Verhoeven¹  Paul Kellam²  Katherine Loens³  Herman Goossens³  Margareta Ieven³  Lia van der Hoek¹ 

[1] Laboratory of Experimental Virology, Department of Medical Microbiology, Center for Infection and Immunity Amsterdam (CINIMA), Academic Medical Center of the University of Amsterdam, Amsterdam 1105 AZ, the Netherlands; E-Mails:;Department of Virus Genomics, Wellcome Trust Sanger Institute, Hinxton CB10 1SA, UK; E-Mails:;Department of Medical Microbiology, Vaccine and Infectious Disease Institute, University Hospital, Antwerp B-2650, Belgium; E-Mails:

关键词: rhinovirus C54;    virus discovery;    antibody capture VIDISCA-454;   

DOI  :  10.3390/v7010239

来源: mdpi

【 摘 要 】

Causative agents for more than 30 percent of respiratory infections remain unidentified, suggesting that unknown respiratory pathogens might be involved. In this study, antibody capture VIDISCA-454 (virus discovery cDNA-AFLP combined with Roche 454 high-throughput sequencing) resulted in the discovery of a novel type of rhinovirus C (RV-C). The virus has an RNA genome of at least 7054 nt and carries the characteristics of rhinovirus C species. The gene encoding viral protein 1, which is used for typing, has only 81% nucleotide sequence identity with the closest known RV-C type, and, therefore, the virus represents the first member of a novel type, named RV-C54.

【 授权许可】

CC BY   
© 2015 by the authors; licensee MDPI, Basel, Switzerland.