Hepatic glucagon receptors expression in response to glucose, insulin, glucagon and immune challenge in grass carp (Ctenophcuyngodon idellus)

Aquaculture Reports

卷:18

Guoxing Nie¹  Hui Cao²  Xiao Yan²  Chaobin Qin²  Xiaolin Meng²  Liping Yang²  Guokun Yang²  Wenli Zhao² 

[1] Corresponding author.;

[2] Engineering Technology Research Center of Henan Province for Aquatic Animal Cultivation, College of Fisheries, Henan Normal University, No. 46 Jianshe Road, Xinxiang 453007, PR China;

关键词: Grass carp;    Glucagon receptor;    Insulin;    Glucagon;    Immune response;   

DOI  :  

来源: DOAJ

【 摘 要 】

Since discovery, glucagon biology has remained enigmatic. Studies have shown that glucagon may have physiological effects beyond glucose regulation by binding to its receptor (Gcgr). The potential roles of glucagon and its receptor in the crosstalk between glucose metabolism and immune function of fish remain to be investigated. In the present study, we sequenced and characterized gcgra and gcgrb in grass carp. Phylogenetic analysis showed that they were highly homologous with their counterparts in fish. The gcgra and gcgrb mRNAs were ubiquitously expressed in all the tissues examined. The gcgra mRNA was highly expressed in the liver and gcgrb was mainly expressed in the liver and brain regions of grass carp. Furthermore, the results of oral glucose tolerance test (OGTT) showed that the expression levels of the two receptors were significantly increased at 12 h after treatment. In the hepatocytes, insulin inhibited gcgra and gcgrb mRNA expressions at 3−12 h and 6−12 h, respectively. While glucagon promoted their expressions at 3−12 h and 3 h, respectively. Intraperitoneal injection of lipopolysaccharide (LPS) increased hepatic gcgra mRNA level at 6 h post treatment, and up-regulated gcgrb mRNA abundance in the liver at 24 h. In response to polyinosinic polycytidylic acid [poly (I:C)] stimuli, however, gcgra and gcgrb mRNA levels were decreased. These results suggested that glucagon receptors were affected by bacterial-like and virus-like infection beyond their roles in the glucose metabolism of grass carp.

【 授权许可】

Unknown