Liquid Chromatography-Tandem Mass Spectrometry of Desoxo-Narchinol a and Its Pharmacokinetics and Oral Bioavailability in Rats and Mice

Molecules

卷:24

TaeHwan Kim¹  Sung-Joo Park²  Soyoung Shin³  SubindraKazi Thapa³  Mahesh Upadhyay³  BeomSoo Shin⁴ 

[1] College of Pharmacy, Daegu Catholic University, Gyeongsan, Gyeongbuk 38430, Korea;

[2] Department of Herbology, School of Korean Medicine, Wonkwang University, Iksan, Jeonbuk 54538, Korea;

[3] Department of Pharmacy, College of Pharmacy, Wonkwang University, Iksan, Jeonbuk 54538, Korea;

[4] School of Pharmacy, Sungkyunkwan University, Suwon, Gyeonggi 16419, Korea;

关键词: desoxo-narchinol A;    Nardostachys jatamansi;    LC-MS/MS;    pharmacokinetics;    bioavailability;   

DOI  :  10.3390/molecules24112037

来源: DOAJ

【 摘 要 】

Desoxo-narchinol A is one of the major active constituents from Nardostachys jatamansi, which has been reported to possess various pharmacological activities, including anti-inflammatory, antioxidant, and anticonvulsant activity. A simple and sensitive liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was developed and validated for the quantification of desoxo-narchinol A in two different biological matrices, i.e., rat plasma and mouse plasma, using sildenafil as an internal standard (IS). The method involved simple protein precipitation with acetonitrile and the analyte was separated by gradient elution using 100% acetonitrile and 0.1% formic acid in water as a mobile phase. The MS detection was performed with a turbo electrospray in positive ion mode. The lower limit of quantification was 10 ng/mL in both rat and mouse plasma. Intra- and inter-day accuracies were in the ranges of 97.23−104.54% in the rat plasma and 95.90−110.11% in the mouse plasma. The precisions were within 8.65% and 6.46% in the rat and mouse plasma, respectively. The method was applied to examine the pharmacokinetics of desoxo-narchinol A, and the oral bioavailability of desoxo-narchinol A was 18.1% in rats and 28.4% in mice. The present results may be useful for further preclinical and clinical studies of desoxo-narchinol A.

【 授权许可】

Unknown