Challenges in Tracking of Fluorochrome-Labelled Nanoparticles in Mice via Whole Body NIRF Imaging

Nanomaterials

卷:10

Martin Kramer¹  Ingrid Hilger²  Ulf Teichgräber²  Andrea Tilch²  Florian Gaffron²  Cordula Grüttner³  Anja Kowalski³ 

[1] Department of Veterinary Clinical Sciences, Small Animal Clinic, Justus- Liebig-University, D-35390 Gießen, Germany;

[2] Institute for Diagnostic and Interventional Radiology, Jena University Hospital—Friedrich Schiller University Jena, D-07740 Jena, Germany;

[3] Micromod Partikeltechnologie GmbH, Friedrich-Barnewitz-Str. 4, D-18119 Rostock, Germany;

关键词: fluorescence;    nanoparticles;    iron oxide;    nanoparticle tracking;    nanoparticle labelling;   

DOI  :  10.3390/nano10030596

来源: DOAJ

【 摘 要 】

Fluorochrome-labelled iron oxide magnetic nanoparticles (MNP) have been of great help in elucidating biological processes. Here, we used dually-fluorochrome-labelled MNP and studied to what extent fluorescence detection could reflect their fate in living animals. One day after application in mice (200 µmol Fe/kg body weight), the fluorescence of the dye attached to the core (DY-730) was very prominent and in agreement with the increase of iron in the liver and spleen of mice, but inconspicuous at time points thereafter. We attribute this fluorescence behavior to early degradation processes of the MNP´s core in the cellular lysosomal compartment. In contrast, the fluorescence of the dye DY-555 stuck to the PEG coating was not detectable in vivo. In summary, labelling of MNP with dyes at their metallic core could be of help when detecting first incidences of MNP biodegradation in vivo, as opposed to dyes attached to the MNP coating.

【 授权许可】

Unknown