Transcriptomic Analysis of Rat Macrophages

Frontiers in Immunology

卷:11

Gemma M. Davis²  Katharine M. Irvine³  David A. Hume³  Stephen J. Bush⁴  Clare Pridans⁵  Lucas Lefevre⁶ 

[1] Centre for Inflammation Research, University of Edinburgh Centre for Inflammation Research, Edinburgh, United Kingdom;

[2] Faculty of Life Sciences, University of Manchester, Manchester, United Kingdom;

[3] Mater Research Institute Mater Research Institute – University of Queensland, Brisbane, QLD, Australia;

[4] Nuffield Department of Clinical Medicine, University of Oxford, Headington, United Kingdom;

[5] Simons Initiative for the Developing Brain, University of Edinburgh, Edinburgh, United Kingdom;

[6] UK Dementia Research Institute, University of Edinburgh, Edinburgh, United Kingdom;

关键词: macrophage;    colony-stimulating factor 1 receptor;    rat;    Kupffer cell;    lipopolysaccharide;   

DOI  :  10.3389/fimmu.2020.594594

来源: DOAJ

【 摘 要 】

The laboratory rat is widely used as a model for human diseases. Many of these diseases involve monocytes and tissue macrophages in different states of activation. Whilst methods for in vitro differentiation of mouse macrophages from embryonic stem cells (ESC) and bone marrow (BM) are well established, these are lacking for the rat. The gene expression profiles of rat macrophages have also not been characterised to the same extent as mouse. We have established the methodology for production of rat ESC-derived macrophages and compared their gene expression profiles to macrophages obtained from the lung and peritoneal cavity and those differentiated from BM and blood monocytes. We determined the gene signature of Kupffer cells in the liver using rats deficient in macrophage colony stimulating factor receptor (CSF1R). We also examined the response of BM-derived macrophages to lipopolysaccharide (LPS). The results indicate that many, but not all, tissue-specific adaptations observed in mice are conserved in the rat. Importantly, we show that unlike mice, rat macrophages express the CSF1R ligand, colony stimulating factor 1 (CSF1).

【 授权许可】

Unknown