Bone-targeted erythrocyte-cancer hybrid membrane-camouflaged nanoparticles for enhancing photothermal and hypoxia-activated chemotherapy of bone invasion by OSCC

Journal of Nanobiotechnology

Jingqing Zhang¹  Yungang He²  Hongying Chen²  Jiang Deng²  Xiaoqing Zhang²  Yi Tang²  Hongwei Dai²  Hanyue Li²  Zhixiang Jian²  Xintong Yao³ 

[1] Chongqing Research Center for Pharmaceutical Engineering, Chongqing Medical University, 400016, Chongqing, China;College of Stomatology, Chongqing Medical University, 401147, Chongqing, China;Chongqing Key Laboratory of Oral Diseases and Biomedical Sciences, 401147, Chongqing, China;Chongqing Municipal Key Laboratory of Oral Biomedical Engineering of Higher Education, 401147, Chongqing, China;Department of Pharmacology, School of Pharmacy, Chongqing Medical University, 400016, Chongqing, China;Key Laboratory of Biochemistry and Molecular Pharmacology of Chongqing, Chongqing Medical University, 400016, Chongqing, China;

关键词: Hybrid membrane;    Homing-targeting;    Bone targeting;    Biomimetic nanoparticle;    Drug delivery;   

DOI  :  10.1186/s12951-021-01088-9

来源: Springer

【 摘 要 】

BackgroundJaw bones are the most common organs to be invaded by oral malignancies, such as oral squamous cell carcinoma (OSCC), because of their special anatomical relationship. Various serious complications, such as pathological fractures and bone pain can significantly decrease the quality of life or even survival outcomes for a patient. Although chemotherapy is a promising strategy for bone invasion treatment, its clinical applications are limited by the lack of tumor-specific targeting and poor permeability in bone tissue. Therefore, it is necessary to develop a smart bone and cancer dual targeting drug delivery platform.ResultsWe designed a dual targeting nano-biomimetic drug delivery vehicle Asp8[H40-TPZ/IR780@(RBC-H)] that has excellent bone and cancer targeting as well as immune escape abilities to treat malignancies in jaw bones. These nanoparticles were camouflaged with a head and neck squamous cell carcinoma WSU-HN6 cell (H) and red blood cell (RBC) hybrid membrane, which were modified by an oligopeptide of eight aspartate acid (Asp8). The spherical morphology and typical core-shell structure of biomimetic nanoparticles were observed by transmission electron microscopy. These nanoparticles exhibited the same surface proteins as those of WSU-HN6 and RBC. Flow cytometry and confocal microscopy showed a greater uptake of the biomimetic nanoparticles when compared to bare H40-PEG nanoparticles. Biodistribution of the nanoparticles in vivo revealed that they were mainly localized in the area of bone invasion by WSU-HN6 cells. Moreover, the Asp8[H40-TPZ/IR780@(RBC-H)] nanoparticles exhibited effective cancer growth inhibition properties when compared to other TPZ or IR780 formulations.ConclusionsAsp8[H40-TPZ/IR780@(RBC-H)] has bone targeting, tumor-homing and immune escape abilities, therefore, it is an efficient multi-targeting drug delivery platform for achieving precise anti-cancer therapy during bone invasion.Graphical Abstract

【 授权许可】

CC BY