Sequential immunization with SARS-CoV-2 RBD vaccine induces potent and broad neutralization against variants in mice

Virology Journal

Yang-Xin Fu¹  Qing Fan²  Bing Zhou²  Lin Cheng²  Xiangyang Ge²  Weilong Liu²  Shuo Song²  Bin Ju³  Zheng Zhang⁴  Hua Peng⁵ 

[1] Department of Basic Medical Sciences, School of Medicine, Tsinghua University, Beijing, China;Institute for Hepatology, National Clinical Research Center for Infectious Disease, Shenzhen Third People’s Hospital, Shenzhen, Guangdong Province, China;The Second Affiliated Hospital, School of Medicine, Southern University of Science and Technology, Shenzhen, Guangdong Province, China;Institute for Hepatology, National Clinical Research Center for Infectious Disease, Shenzhen Third People’s Hospital, Shenzhen, Guangdong Province, China;The Second Affiliated Hospital, School of Medicine, Southern University of Science and Technology, Shenzhen, Guangdong Province, China;Guangdong Key Laboratory for Anti-Infection Drug Quality Evaluation, Shenzhen, Guangdong Province, China;Institute for Hepatology, National Clinical Research Center for Infectious Disease, Shenzhen Third People’s Hospital, Shenzhen, Guangdong Province, China;The Second Affiliated Hospital, School of Medicine, Southern University of Science and Technology, Shenzhen, Guangdong Province, China;Shenzhen Research Center for Communicable Disease Diagnosis and Treatment of Chinese Academy of Medical Science, Shenzhen, Guangdong Province, China;Guangdong Key Laboratory for Anti-Infection Drug Quality Evaluation, Shenzhen, Guangdong Province, China;Institute of Biophysics, Chinese Academy of Sciences, Beijing, China;Guangzhou Laboratory, and Bioland Laboratory (Guangzhou Regenerative Medicine and Health Guangdong Laboratory), Guangzhou, China;

关键词: SARS-CoV-2 variant;    Inactivated vaccine;    Mutant RBD vaccine;    Sequential immunization;    Broadly neutralizing antibody;   

DOI  :  10.1186/s12985-021-01737-3

来源: Springer

【 摘 要 】

The current COVID-19 pandemic caused by constantly emerging SARS-CoV-2 variants still poses a threat to public health worldwide. Effective next-generation vaccines and optimized booster vaccination strategies are urgently needed. Here, we sequentially immunized mice with a SARS-CoV-2 wild-type inactivated vaccine and a heterologous mutant RBD vaccine, and then evaluated their neutralizing antibody responses against variants including Beta, Delta, Alpha, Iota, Kappa, and A.23.1. These data showed that a third booster dose of heterologous RBD vaccine especially after two doses of inactivated vaccines significantly enhanced the GMTs of nAbs against all SARS-CoV-2 variants we tested. In addition, the WT and variants all displayed good cross-immunogenicity and might be applied in the design of booster vaccines to induce broadly neutralizing antibodies.

【 授权许可】

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