Carriage of distinct blaKPC-2 and blaOXA-48 plasmids in a single ST11 hypervirulent Klebsiella pneumoniae isolate in Egypt

BMC Genomics

Lingqing Xu¹  Weihong Wen¹  Reem Mostafa Hassan²  Yiping Wu³  Yanxian Yang³  Lan-Lan Zhong³  Ruowen He³  Mohamed Abd El-Gawad El-Sayed Ahmed⁴  Lin Xu⁵  Guo-Bao Tian⁶  Yongqiang Yang⁷  Baoguo Deng⁸  Mingyang Qin⁸  Ping Chen⁸  Xubin Huang⁹  Xiaoxue Liang^1,10 

[1] Department of Clinical Laboratory, The Sixth Affiliated Hospital of Guangzhou Medical University, Qingyuan People’s Hospital, 511518, Qingyuan, China;Department of Clinical and Chemical Pathology, Faculty of Medicine, Cairo University, Cairo, Egypt;Department of Microbiology, Zhongshan School of Medicine, Sun Yat-sen University, 74 Zhongshan 2nd Road, 510080, Guangzhou, China;Key Laboratory of Tropical Diseases Control (Sun Yat-sen University), Ministry of Education, 510080, Guangzhou, China;Department of Microbiology, Zhongshan School of Medicine, Sun Yat-sen University, 74 Zhongshan 2nd Road, 510080, Guangzhou, China;Key Laboratory of Tropical Diseases Control (Sun Yat-sen University), Ministry of Education, 510080, Guangzhou, China;Department of Microbiology and Immunology, Faculty of Pharmaceutical Sciences and Drug Manufacturing, Misr University for Science and Technology, Cairo, 6th of October City, Egypt;Department of Microbiology, Zhongshan School of Medicine, Sun Yat-sen University, 74 Zhongshan 2nd Road, 510080, Guangzhou, China;Key Laboratory of Tropical Diseases Control (Sun Yat-sen University), Ministry of Education, 510080, Guangzhou, China;Research Center for Clinical Laboratory Standard, Zhongshan School of Medicine, Sun Yat⁃sen University, Guangzhou, China;Department of Microbiology, Zhongshan School of Medicine, Sun Yat-sen University, 74 Zhongshan 2nd Road, 510080, Guangzhou, China;Key Laboratory of Tropical Diseases Control (Sun Yat-sen University), Ministry of Education, 510080, Guangzhou, China;School of Medicine, Xizang Minzu University, 712082, Xianyang, Shaanxi, China;Department of Microbiology, Zhongshan School of Medicine, Sun Yat-sen University, 74 Zhongshan 2nd Road, 510080, Guangzhou, China;Key Laboratory of Tropical Diseases Control (Sun Yat-sen University), Ministry of Education, 510080, Guangzhou, China;School of Pharmaceutical Sciences (Shenzhen), Sun Yat-sen University, 510006, Guangzhou, China;Department of Pathogen Biology, School of Basic Medical, Xinxiang Medical University, 453003, Xinxiang, China;Department of Pulmonary and Critical Care Medicine, the First Affiliated Hospital, Sun Yat-sen University, Guangzhou, China;School of Laboratory Medicine, Chengdu Medical College, 610500, Chengdu, China;

关键词: K. pneumoniae;    KPC-2;    OXA-48;    Hypervirulence;    Egypt;   

DOI  :  10.1186/s12864-021-08214-9

来源: Springer

【 摘 要 】

BackgroundCarbapenem-resistant hypervirulent K. pneumoniae (CR-hvKP) causes serious infections with significant morbidity and mortality. However, the epidemiology and transmission mechanisms of CR-hvKP and the corresponding carbapenem-resistant plasmids require further investigation. Herein, we have characterized an ST11 K. pneumoniae strain EBSI041 from the blood sample encoding both hypervirulence and carbapenem resistance phenotypes from a patient in Egypt.ResultsK. pneumoniae strain EBSI041 showed multidrug-resistance phenotypes, where it was highly resistant to almost all tested antibiotics including carbapenems. And hypervirulence phenotypes of EBSI041 was confirmed by the model of Galleria mellonella infection. Whole-genome sequencing analysis showed that the hybrid plasmid pEBSI041-1 carried a set of virulence factors rmpA, rmpA2, iucABCD and iutA, and six resistance genes aph(3′)-VI, armA, msr(E), mph(E), qnrS, and sul2. Besides, blaOXA-48 and blaSHV-12 were harboured in a novel conjugative IncL-type plasmid pEBSI041-2. The blaKPC-2-carrying plasmid pEBSI041-3, a non-conjugative plasmid lacking the conjugative transfer genes, could be transferred with the help of pEBSI041-2, and the two plasmids could fuse into a new plasmid during co-transfer. Moreover, the emergence of the p16HN-263_KPC-like plasmids is likely due to the integration of pEBSI041-3 and pEBSI041-4 via IS26-mediated rearrangement.ConclusionTo the best of our knowledge, this is the first report on the complete genome sequence of KPC-2- and OXA-48-coproducing hypervirulent K. pneumoniae from Egypt. These results give new insights into the adaptation and evolution of K. pneumoniae during nosocomial infections.

【 授权许可】

CC BY