Salivary anti-nuclear antibody (ANA) mirrors serum ANA in systemic lupus erythematosus

Arthritis Research & Therapy

Qingqing Wu¹  Yong Du¹  Valeria Duran¹  Hao Li¹  Chandra Mohan¹  Thao Nguyen¹  Gabriel Gidley¹  Ting Zhang²  Daniel Goldman³  Michelle Petri³ 

[1] Biomedical Engineering Department, University of Houston, 3517 Cullen Blvd, Room 2027, Houston, TX, USA;Biomedical Engineering Department, University of Houston, 3517 Cullen Blvd, Room 2027, Houston, TX, USA;Present affiliation: The Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, China;Division of Rheumatology, Johns Hopkins University School of Medicine, Baltimore, MD, USA;

关键词: SLE;    Saliva;    ANA;    Isotype;   

DOI  :  10.1186/s13075-021-02694-6

来源: Springer

【 摘 要 】

ObjectivesTo assay salivary anti-nuclear antibody (ANA) and its isotypes in patients with systemic lupus erythematosus (SLE) and to investigate relevant clinical associations.MethodsSaliva samples were collected from SLE patients and assayed for salivary ANA using immunofluorescence (IF). Isotypes of salivary ANA, including IgG-ANA, IgA-ANA, and IgM-ANA, were quantified using enzyme-linked immunosorbent assay. The correlations between clinical parameters and levels of salivary ANA and isotypes were evaluated.ResultsSalivary ANA IF intensities were significantly higher in SLE patients than in healthy controls, irrespective of SLE patient disease activity, and strongly correlated with serum ANA titers. Salivary ANA was detected in 67.14% of SLE patients and 10.00% of healthy controls (p < 0.001). Among ANA-positive samples, 80.85% exhibited a nuclear ANA pattern, and 42.55% exhibited a cytoplasmic ANA pattern. Salivary IgG-ANA, IgA-ANA, and IgM-ANA levels, as assayed by ELISA, were significantly increased in both active and less active SLE patients compared with healthy controls, and levels of each isotype were significantly correlated with serum ANA titer. Salivary IgM-ANA levels correlated with the physician global assessment (PGA), SLE disease activity index (SLEDAI), and negatively with serum C3 and C4. Salivary IgG-ANA also correlated with erythrocyte sedimentation rate (ESR), SLEDAI, and negatively with serum C3.ConclusionSalivary ANA levels correlate with serum ANA titer, and salivary IgM-ANA and IgG-ANA correlate variably with PGA, SLEDAI, ESR and complement levels. These findings underscore the potential of using salivary ANA and ANA isotypes as surrogates for serum ANA, particularly for future point-of-care applications since saliva is easier to obtain than blood.

【 授权许可】

CC BY