Molecular mass analysis of murine immunosuppressive immunoglobulin G‐binding factors (IgG‐BFs) produced by T‐cell hybrids

FEBS Letters

Gelabert, Maria-José¹  Fridman, Wolf Herman¹  Moncuit, Janine¹  Néauport-Sautès, Catherine¹ 

[1] Unité INSERM 255, Institut Curie, 26, rue d'Ulm, 75231 Paris Cédex 05, France

关键词: Molecular mass;    T-cell hybrid;    T-cell factor;    Antibody production;    Isotype;    Polyacrylamide gel electrophoresis;   

DOI  :  10.1016/0014-5793(84)80456-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Induced and constitutive murine IgG-binding factors (IgG-BFs) have been purified by affinity chromatography from supernatants of T-cells preincubated with or without murine monoclonal IgG1 and IgG2b, respectively. IgG-BF M _r values have been studied by SDS-polyacrylamide gel electrophoresis (PAGE) after treatment with SDS under conditions which do not noticeably alter their immunosuppressive activities on the secondary in vitro IgG antibody response. Suppression was recovered at M _r values of 80000, 40000 and 20000. When induced IgG-BF was tested, the isotype-specific suppressive activity was found only at 40 kDa. The 20-kDa moiety appeared to derive from the 40-kDa component and the material found at 80 kDa exerted non-specific immunosuppressive effects. We conclude therefore that isotype-specific IgG-BF has an apparent M _r of 40000.

【 授权许可】

Unknown