期刊论文详细信息
Journal of Nanobiotechnology
Phenotypic change of mesenchymal stem cells into smooth muscle cells regulated by dynamic cell-surface interactions on patterned arrays of ultrathin graphene oxide substrates
Jin-Ho Lee1  Suck Won Hong2  Rowoon Park2  Jae Ho Kim3  Jung Won Yoon3 
[1] Department of Biomedical Convergence Engineering, Pusan National University, 50612, Yangsan, Republic of Korea;Department of Cogno-Mechatronics Engineering, Pusan National University, 46241, Busan, Republic of Korea;Department of Physiology, School of Medicine, Pusan National University, 50612, Yangsan, Republic of Korea;
关键词: Stem cells;    Self-assembly;    Lithography;    Tissue engineering;    Smooth muscle cells;   
DOI  :  10.1186/s12951-021-01225-4
来源: Springer
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【 摘 要 】

The topographical interface of the extracellular environment has been appreciated as a principal biophysical regulator for modulating cell functions, such as adhesion, migration, proliferation, and differentiation. Despite the existed approaches that use two-dimensional nanomaterials to provide beneficial effects, opportunities evaluating their impact on stem cells remain open to elicit unprecedented cellular responses. Herein, we report an ultrathin cell-culture platform with potential-responsive nanoscale biointerfaces for monitoring mesenchymal stem cells (MSCs). We designed an intriguing nanostructured array through self-assembly of graphene oxide sheets and subsequent lithographical patterning method to produce chemophysically defined regions. MSCs cultured on anisotropic micro/nanoscale patterned substrate were spontaneously organized in a highly ordered configuration mainly due to the cell-repellent interactions. Moreover, the spatially aligned MSCs were spontaneously differentiated into smooth muscle cells upon the specific crosstalk between cells. This work provides a robust strategy for directing stem cells and differentiation, which can be utilized as a potential cell culture platform to understand cell–substrate or cell–cell interactions, further developing tissue repair and stem cell-based therapies.Graphical Abstract

【 授权许可】

CC BY   

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