Circular RNA circCCDC85A inhibits breast cancer progression via acting as a miR-550a-5p sponge to enhance MOB1A expression

Breast Cancer Research

Yang Sang¹  Pingan Ding²  Sheng Chang³  Fei Liu³  Ziyi Li³  Liuxin Wang³  Xixi Nan³  Ruiyu Xu³  Lina Gu³  Yang Zheng⁴  Lingjiao Meng⁴  Meixiang Sang⁴ 

[1] Animal Center, The Fourth Hospital of Hebei Medical University, 050017, Shijiazhuang, Hebei, People’s Republic of China;The Third Department of Surgery, The Fourth Hospital of Hebei Medical University, 050017, Shijiazhuang, Hebei, People’s Republic of China;Tumor Research Institute, The Fourth Affiliated Hospital of Hebei Medical University, 050017, Shijiazhuang, Hebei, People’s Republic of China;Tumor Research Institute, The Fourth Affiliated Hospital of Hebei Medical University, 050017, Shijiazhuang, Hebei, People’s Republic of China;Research Center, The Fourth Hospital of Hebei Medical University, 050017, Shijiazhuang, Hebei, People’s Republic of China;

关键词: Breast cancer;    Circular RNA;    circCCDC85A;    miR-550a-5p;    MOB1A;   

DOI  :  10.1186/s13058-021-01497-6

来源: Springer

【 摘 要 】

BackgroundA growing body of evidence indicates that abnormal expression of circular RNAs (circRNAs) plays a crucial role by acting as molecular sponges of microRNAs (miRNAs) in various diseases, including cancer. In this study, we explored whether circCCDC85A could function as a miR-550a-5p sponge and influence breast cancer progression.MethodsWe detected the expression of circCCDC85A in breast cancer tissues and cells using fluorescence in situ hybridization (FISH) and quantitative reverse transcription polymerase chain reaction (qRT-PCR). CCK-8 and colony formation assay were used to detect the proliferative ability of breast cancer cells. Wound healing assay and transwell migration and invasion assays were used to detect the migrative and invasive abilities of breast cancer cells. We also examined the interactions between circCCDC85A and miR-550a-5p using FISH, RNA-binding protein immunoprecipitation (RIP), and luciferase reporter assay. Moreover, we performed luciferase reporter assay, qRT-PCR, and Western blot to confirm the direct targeting of miR-550a-5p to MOB1A.ResultsThe expression of circCCDC85A in breast cancer tissues was obviously lower than that in normal breast tissues. Over-expression of circCCDC85A substantially inhibited the proliferative, migrative, and invasive ability of breast cancer cells, while knocking down of circCCDC85A enhanced the aforementioned properties of breast cancer cells. Moreover, enforced expression of circCCDC85A inhibits the oncogenic activity of miR-550a-5p and increases the expression of MOB1A targeted by miR-550a-5p. Further molecular mechanism research showed that circCCDC85A may act as a molecular sponge for miR-550a-5p, thus restoring miR-550a-5p-mediated targeting repression of tumor suppressor MOB1A in breast cancer cells.ConclusionOur findings provide novel evidence that circCCDC85A inhibits the progression of breast cancer by functioning as a molecular sponge of miR-550a-5p to enhance MOB1A expression.

【 授权许可】

CC BY