Weight-loss in obese dogs promotes important shifts in fecal microbiota profile to the extent of resembling microbiota of lean dogs

Animal Microbiome

Thiago Henrique Annibale Vendramini¹  Mariana Fragoso Rentas¹  Andressa Rodrigues Amaral¹  Júlio César de Carvalho Balieiro¹  Marcio Antonio Brunetto¹  Henrique Tobaro Macedo¹  Matheus Vinicius Macegoza¹  Eduardo Ferriolli²  Karina Pfrimer²  Juliana Toloi Jeremias³  Cristiana Ferreira Fonseca Pontieri³ 

[1] Department of Animal Nutrition and Production, Pet Nutrology Research Center (CEPEN Pet), School of Veterinary Medicine and Animal Science (FMVZ), University of São Paulo (USP), Duque de Caxias Norte Ave, 255, 13635-900, Pirassununga, São Paulo, Brazil;Medical School of Ribeirão Preto (FMRP), University of São Paulo (USP), Bandeirantes Ave, 3900 - Campus da USP, 14049-900, Ribeirão Preto, São Paulo, Brazil;Nutritional Development Center (CDN), Grandfood Indústria E Comércio LTDA (Premier Pet), Luiz Augusto de Oliveira Hwy, Km 204, 13590-000, Dourado, São Paulo, Brazil;

关键词: Canine;    Dysbiosis;    Gastrointestinal tract;    Microbiome;    Obesity;   

DOI  :  10.1186/s42523-021-00160-x

来源: Springer

【 摘 要 】

BackgroundAmong the undesirable changes associated with obesity, one possibility recently raised is dysbiosis of the intestinal microbiota. Studies have shown changes in microbiota in obese rats and humans, but there are still few studies that characterize and compare the fecal microbiota of lean, obese and dogs after weight loss. Thus, this study aimed to evaluate the effects of a weight loss program (WLP) in fecal microbiota of dogs in addition to comparing them with those of lean dogs. Twenty female dogs of different breeds, aged between 1 and 9 years were selected. They were equally divided into two groups: Obese group (OG), with body condition score (BCS) 8 or 9/9, and body fat percentage greater than 30%, determined by the deuterium isotope dilution method, and lean group (LG) with BCS 5/9, and maximum body fat of 15%. Weight loss group (WLG) was composed by OG after loss of 20% of their current body weight. Fecal samples were collected from the three experimental groups. Total DNA was extracted from the feces and these were sequenced by the Illumina methodology. The observed abundances were evaluated using a generalized linear model, considering binomial distribution and using the logit link function in SAS (p < 0.05).ResultsThe WLP modulated the microorganisms of the gastrointestinal tract, so that, WLG and LG had microbial composition with greater biodiversity than OG, and intestinal uniformity of the microbiota (Pielou’s evenness index) was higher in OG than WLG dogs (P = 0.0493) and LG (P = 0.0101). In addition, WLG had values of relative frequency more similar to LG than to OG.ConclusionThe fecal microbiota of the studied groups differs from each other. The weight loss program can help to reverse the changes observed in obese dogs.

【 授权许可】

CC BY