The industrial solvent 1,4-dioxane causes hyperalgesia by targeting capsaicin receptor TRPV1

BMC Biology

Dongdong Li¹  Yue Gao²  Quan Tian²  Bo Zhong²  Chang Xie²  Youjing Zhang²  Yuanyuan Wang²  Peiyuan Pang²  Xiaoyi Mo²  Tianchen Xiong²  Xin Wei²  Luna Gao²  Qiang Liu²  Jing Yao² 

[1] Institute of Biology Paris Seine, Neuroscience Paris Seine, Sorbonne Université, CNRS UMR8246, INSERM U1130, UPMC UM119, 75005, Paris, France;State Key Laboratory of Virology, College of Life Sciences, Department of Anesthesiology, Zhongnan Hospital of Wuhan University, Frontier Science Center for Immunology and Metabolism, Wuhan University, 430072, Wuhan, Hubei, China;

关键词: TRPV1;    Hyperalgesia;    1,4-Dioxane;    Nociception;    Inflammatory pain;   

DOI  :  10.1186/s12915-021-01211-0

来源: Springer

【 摘 要 】

BackgroundThe synthetic chemical 1,4-dioxane is used as industrial solvent, food, and care product additive. 1,4-Dioxane has been noted to influence the nervous system in long-term animal experiments and in humans, but the molecular mechanisms underlying its effects on animals were not previously known.ResultsHere, we report that 1,4-dioxane potentiates the capsaicin-sensitive transient receptor potential (TRP) channel TRPV1, thereby causing hyperalgesia in mouse model. This effect was abolished by CRISPR/Cas9-mediated genetic deletion of TRPV1 in sensory neurons, but enhanced under inflammatory conditions. 1,4-Dioxane lowered the temperature threshold for TRPV1 thermal activation and potentiated the channel sensitivity to agonistic stimuli. 1,3-dioxane and tetrahydrofuran which are structurally related to 1,4-dioxane also potentiated TRPV1 activation. The residue M572 in the S4-S5 linker region of TRPV1 was found to be crucial for direct activation of the channel by 1,4-dioxane and its analogs. A single residue mutation M572V abrogated the 1,4-dioxane-evoked currents while largely preserving the capsaicin responses. Our results further demonstrate that this residue exerts a gating effect through hydrophobic interactions and support the existence of discrete domains for multimodal gating of TRPV1 channel.ConclusionsOur results suggest TRPV1 is a co-receptor for 1,4-dioxane and that this accounts for its ability to dysregulate body nociceptive sensation.

【 授权许可】

CC BY