Circular RNA ciRs-126 promotes hypoxia/reoxygenation cardiac injury possibly through miR-21

Thrombosis Journal

Changming Tan¹  Zhaoshun Yuan¹  Jianming Li¹  Yongxin Mu² 

[1] Department of Cardiovascular Surgery, The Second Xiangya Hospital, Central South University, No.139 Middle Renmin Road, 410011, Changsha, Hunan, China;Department of Medicine, University of California, La Jolla, San Diego, CA, USA;

关键词: Hypoxia/reoxygenation cardiac injury;    ciRs-126;    miR-21;    Apoptosis;   

DOI  :  10.1186/s12959-021-00355-x

来源: Springer

【 摘 要 】

BackgroundThis study aimed to analyze the role of circular RNA ciRs-126 in hypoxia/reoxygenation cardiac injury (H/R).MethodsExpression of ciRs-126 and miR-21 in plasma samples from patients with H/R and healthy controls was determined by RT-qPCR. Correlations were analyzed by linear regression. Overexpression of ciRs-126 and miR-21 was achieved in cardiomyocytes to explore their crosstalk. The roles of ciRs-126 and miR-21 in H/R-induced apoptosis of cardiomyocytes were analyzed using cell apoptosis assay.ResultsCiRs-126 was upregulated and miR-21 was downregulated in H/R patients. They were inversely correlated across plasma samples from H/R patients. In H/R cardiomyocytes, ciRs-126 was upregulated and miR-21 was downregulated. In cardiomyocytes, ciRs-126 overexpression decreased miR-21 level and reduced the inhibitory effects of miR-21 overexpression on H/R-induced cell apoptosis.ConclusionsCircular RNA ciRs-126 may suppress miR-21 expression to promote H/R cardiac injury.

【 授权许可】

CC BY