Identification of crucial genes of pyrimidine metabolism as biomarkers for gastric cancer prognosis

Cancer Cell International

Yuekun Zhu¹  Zhijian Ye²  Mengya Zhong³  Jinshui Tan³  Haijie Lu⁴  Huiwen Zhou⁵  Yubo Xiong⁵  Yifan Zhuang⁵  Jingsong Ma⁵  Jiabao Zhao⁵  Zhi Gao⁶  Yan Yang⁷  Zhengxin Wu⁸  Xuehui Hong⁹ 

[1] Department of Colorectal Surgery, The First Affiliated Hospital of Harbin Medical University, 150001, Harbin, Heilongjiang, China;Department of Gastrointestinal Surgery, Zhongshan Hospital, Xiamen University, No. 201-209 Hubin South Road, 361004, Xiamen, Fujian, China;National Center for International Research of Biological Targeting Diagnosis and Therapy, Guangxi Medical University, 530000, Nanning, China;Department of Hematology, The First Affiliated Hospital of Xiamen University and Institute of Hematology, School of Medicine, Xiamen University, 361003, Xiamen, China;Department of Radiation Oncology, Affiliated Zhongshan Hospital of Xiamen University, 361102, Xiamen, China;Institute of Gastrointestinal Oncology, School of Medicine, Xiamen University, 361000, Xiamen, China;Department of Gastrointestinal Surgery, Zhongshan Hospital, Xiamen University, No. 201-209 Hubin South Road, 361004, Xiamen, Fujian, China;National Center for International Research of Biological Targeting Diagnosis and Therapy, Guangxi Medical University, 530000, Nanning, China;Organ Transplantation Institute of Xiamen University, Fujian Provincial Key Laboratory of Organ and Tissue Regeneration, School of Medicine, Xiamen University, Xiang An South Road, 361102, Xiamen, China;School of Medicine, Guangxi University, 530004, Nanning, China;School of Medicine, Guangxi University, 530004, Nanning, China;Institute of Gastrointestinal Oncology, School of Medicine, Xiamen University, 361000, Xiamen, China;Department of Gastrointestinal Surgery, Zhongshan Hospital, Xiamen University, No. 201-209 Hubin South Road, 361004, Xiamen, Fujian, China;

关键词: Pyrimidine metabolism;    GC;    Bioinformatics;    Prognosis risk model;    Biomarker;   

DOI  :  10.1186/s12935-021-02385-x

来源: Springer

【 摘 要 】

BackgroundMetabolic reprogramming has been reported in various kinds of cancers and is related to clinical prognosis, but the prognostic role of pyrimidine metabolism in gastric cancer (GC) remains unclear.MethodsHere, we employed DEG analysis to detect the differentially expressed genes (DEGs) in pyrimidine metabolic signaling pathway and used univariate Cox analysis, Lasso-penalizes Cox regression analysis, Kaplan–Meier survival analysis, univariate and multivariate Cox regression analysis to explore their prognostic roles in GC. The DEGs were experimentally validated in GC cells and clinical samples by quantitative real-time PCR.ResultsThrough DEG analysis, we found NT5E, DPYS and UPP1 these three genes are highly expressed in GC. This conclusion has also been verified in GC cells and clinical samples. A prognostic risk model was established according to these three DEGs by Univariate Cox analysis and Lasso-penalizes Cox regression analysis. Kaplan–Meier survival analysis suggested that patient cohorts with high risk score undertook a lower overall survival rate than those with low risk score. Stratified survival analysis, Univariate and multivariate Cox regression analysis of this model confirmed that it is a reliable and independent clinical factor. Therefore, we made nomograms to visually depict the survival rate of GC patients according to some important clinical factors including our risk model.ConclusionIn a word, our research found that pyrimidine metabolism is dysregulated in GC and established a prognostic model of GC based on genes differentially expressed in pyrimidine metabolism.

【 授权许可】

CC BY